淫羊藿素对人脐带间充质干细胞增殖与细胞因子分泌的影响  

Effect of Icaritin on Proliferation and Cytokine Expression of Human Umbilical Cord Mesenchymal Stem Cells in Vitro

作　　者：张爱娟[1,2] 王汉裕[2,3] 许放 纪意纯[2,4] 邝卫红[2] 

机构地区：[1]青岛市口腔医院,山东青岛266001 [2]广州中医药大学,广东广州510006 [3]天津市干细胞再生医学转化企业重点实验室,天津300300 [4]深圳市宝安区中医院,广东深圳518010

出　　处：《中药新药与临床药理》2017年第2期159-164,共6页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的探索淫羊藿素对人脐带间充质干细胞(UC-MSC)体外增殖及细胞因子分泌的影响。方法分离、培养人脐带来源的间充质干细胞(MSC),采用含不同浓度淫羊藿素的培养基培养,流式细胞术检测MSC表面标志物,Brd U法检测MSC的增殖能力,实时荧光定量PCR法检测MSC分泌细胞生长因子的变化。结果不同浓度(10,20,50,100 ng·mL^(-1))的淫羊藿素均可促进MSC增殖(P<0.05),其中以20,50 ng·mL^(-1)组促增殖作用最明显;与对照组比较,淫羊藿素对尿激酶纤溶酶原激活物(u PA,10 ng·mL-)1、基质金属蛋白酶-1(MMP-1,10,20,50 ng·mL-)1、血管内皮生长因子(VEGF,50 ng·mL^(-1))mR NA的表达明显增加,差异有统计学意义(P<0.05)。结论淫羊藿素可促进UC-MSC体外增殖,并且促进肝细胞生长因子(HGF)、u PA、MMP-1、VEGF的表达。Objective To investigate the effects of icariin on the proliferation and secretion of cytokines of human umbilical cord mesenchymal stem cells（UC-MSC ） in vitro. Methods UC-MSC were isolated and cultured, and then were incubated with medium containing different concentrations of icaritin. We detected UC-MSC surface markers by flow cytometry, examined the proliferation ability by BrdU method, and observed the changes of UC-MSC cytokines by real-time quantitative PCR. Results Icaritin at different concentrations（ 10, 20, 50, 100 ng＇mL-1） could promote the proliferation of UC-MSC（P 〈 0.05）, and the effect of 50 ng·mL-1 group was the most strongest. Compared with the control group, icaritin could promote the mRNA expression of urokinase type plasminogen activator （uPA; icariin in 10 ng·mL-1） , matrix metalloproteinase-1 （MMP-1; icaritin in 10, 20, 50 ng·mL-1） , and vascular endothelial growth factor （VEGF; icaritin in 50 ng·mL-1） in UC-MSC, the differences being significant（P 〈 0.05）. Conclusion Icaritin can promote the proliferation of UC-MSC in vitro, and enhance the expression of cytokines （uPA, MMP-1, VEGF）.

关 键 词：淫羊藿素 间充质干细胞 增殖 肝细胞生长因子 尿激酶型纤溶酶原激活物 基质金属蛋白酶-1 血管内皮生长因子 

分 类 号：R722.12[医药卫生—儿科]