HPLC法测定清热暗疮丸中绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯含量  

HPLC Determination of Chlorogenic Acid,Geniposide,Andrographolide and Dehydroandrographolide in Qingre Anchuang Pills

作　　者：唐德智 严秀荣[2] 

机构地区：[1]广西南宁食品药品检验所,广西南宁530021 [2]广西医科大学第一附属医院,广西南宁530021

出　　处：《中药新药与临床药理》2017年第2期223-227,共5页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立HPLC法测定清热暗疮丸中绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯的含量。方法色谱柱为Agilent Eclipse XDB-C_(18)(4.6 mm×150 mm,5μm);流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱;流速为1.0 m L·min^(-1);柱温:30℃;波长切换时间序列采样:0~7 min为327 nm,7~12 min为238 nm,12~19 min为225 nm,19~40 min为254 nm。结果绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯分别在0.188~3.754μg(r=0.999 8)、0.164~3.272μg(r=0.999 8)、0.020~0.392(r=0.999 9)μg和0.039~0.777μg(r=0.999 9)范围内线性关系良好。平均回收率分别为99.53%,RSD为1.7%(n=9);99.33%,RSD为1.5%(n=9);99.59%,RSD为1.8%(n=9)和99.46%,RSD为1.5%(n=9)。5批清热暗疮丸中绿原酸、栀子苷、穿心莲内酯和脱水穿心莲内酯含量分别为9.48,8.05,0.92,1.92 mg·g^(-1)。结论该方法准确、快捷、可靠,可更好地用于清热暗疮丸的质量控制。Objective To establish a HPLC method for simultaneous determination of chlorogenic acid,geniposide,andrographolide and dehydroandrographolide in Qingre Anchuang Pills. Methods Agilent Eclipse XDB-C18（4.6×150mm,5 μm）column was used. The mobile phase was acetonitrile（A）-water containing 0.1 % phosphoric acid（B）with gradient elution mode at the flow rate of 1.0 m L·min-1. The detection wavelength was 327 nm（0-7 min）for chlorogenic acid,238 nm（7-12 min） for geniposide,225 nm（12-19 min） for andrographolide,254 nm（19-40 min） for dehydroandrographolide. Results Chlorogenic acid had a good linearity in the range of 0.188-3.754 μg, and the average recovery was 99.53 % with RSD being 1.7 %（n=9）. Geniposide had a good linearity in the range of 0.164-3.272 μg,and the average recovery was 99.33 % with RSD being 1.5 %（n=9）. Andrographolide had a good linearity in the range of 0.020-0.392 μg, and the average recovery was 99.59 % with RSD being 1.8 %（n=9）.Dehydroandrographolide had a good linearity in the range of 0.039-0.777 μg,and the average recovery was 99.46 %with RSD being 1.5 %（n=9）. The contents of chlorogenic acid,geniposide,andrographolide and dehydroandrographolide in 5 batches of Qingre Anchuang Pills were 9.48,8.05,0.92,1.92 mg·g（-1）respectively. Conclusion The established method is simple,accurate and reproducible. It can be used for the quality control of Qingre Anchuang Pills.

关 键 词：清热暗疮丸 绿原酸 栀子苷 穿心莲内酯 脱水穿心莲内酯 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]