宫颈局部细胞因子在宫颈持续HR-HPV感染中的表达及意义  

Expression and significance of cervical local cytokines in cervical persistent HR-HPV infection

作　　者：宋华林[1] 张玲[1] 曲芃芃[2] 

机构地区：[1]天津医科大学,天津300070 [2]天津市中心妇产科医院,天津300100

出　　处：《中国妇幼保健》2017年第6期1151-1154,共4页Maternal and Child Health Care of China

基　　金：天津市卫生行业重点攻关项目(15KG140);天津市科技计划项目(09ZCZDSF03900)

摘　　要：目的探讨促炎因子:白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α);趋化因子:IL-8;Th1:IL-2、IL-12、干扰素(IFN)-γ;Th2:IL-10、IL-4在宫颈持续HPV感染免疫微环境中的表达及意义。方法用蛋白芯片技术检测20例HPV一过性感染者、63例HPV持续感染者22例慢性宫颈炎、19列子宫颈低级别鳞状上皮内病变(LSIL)、22例子宫颈高级别鳞状上皮内病变(HSIL),阴道灌洗液中IL-2、IL-12、IL-1β、IL-8、IL-10、IL-4、TNF-α、IFN-γ的表达。结果 TNF-α及IL-1β在一过性感染组中的表达低于HPV持续组(P<0.001、P=0.035),且TNF-α在慢性炎症组、LSIL及HSIL组中的表达均高于一过性感染组(P<0.05);IL-10在一过性感染组中的表达高于慢性炎症组及LSIL(P=0.007、P=0.013),而在LISL中的表达低于HSIL(P=0.027);IL-8在LSIL中的表达低于HSIL(P=0.039);IL-2、IL-12及IFN-γ在各组间表达无统计学意义。结论促炎因子IL-1β、TNF-α及趋化因子IL-8在HPV持续感染或宫颈病变过程中表达增加,为HPV持续性感染及宫颈病变的发生发展提供有利的免疫微环境;IL-4可调节宫颈局部促炎及抗炎反应的平衡;IL-10可能在HPV感染及宫颈病变中不同阶段起着不同的作用。Objective To explore the expressions and significance of IL-1β,tumor necrosis factor-α（TNF-α）,IL-8,IL-2,IL-12,interferon-γ（IFN-γ）,IL-10,and IL-4 in immune microenvironment of cervical persistent HPV infection.Methods Twenty transient HPV infection patients and 63 patients with persistent HPV infection [22 patients with chronic cervicitis and 19 patients with low-grade squamous intraepithelial lesion（LSIL）,and 22 patients with high-grade squamous intraepithelial lesion（HSIL） ] were detected by protein-chip technique.The expressions of IL-2,IL-12,IL-1β,IL-8,IL-10,IL-4,TNF-α,and IFN-γ in vaginal lavage fluid were detected.Results The expressions of TNF-α and IL-1β in HPV transient infection group were statistically significantly lower than those in persistent HPV infection group（P〈0.001,P = 0.035）;the expressions of TNF-α in chronic cervicitis group,LSIL group,and HSIL group were statistically significantly higher than those in HPV transient infection group（P〈0.05）;the expression of IL-10 in HPV transient infection group was statistically significantly higher than those in chronic cervicitis group and LSIL group（P = 0.007,P = 0.013）,but the expression of IL-10 in LSIL group was statistically significantly lower than that in HSIL group（P = 0.027）;the expression of IL-8 in LSIL group was statistically significantly lower than that in HSIL group（P = 0.039）;there was no statistically significant difference in the expressions of IL-2,IL-12,and IFN-γ among different groups.Conclusion Proinflammatory cytokines（IL-1β,TNF-α） and chemokine（IL-8） expressions in HPV persistent infection and cervical lesion increased,which provides a favorable immune microenvironment for HPV persistent infection and the occurrence and development of cervical lesions;IL-4 can regulate the balance of cervical pro-inflammation and anti-inflammation;IL-10 may play different roles in different stages of HPV infection and cervical lesions.

关 键 词：宫颈持续HPV感染 高危型HPV 蛋白芯片技术 细胞因子 免疫微环境 

分 类 号：R711.74[医药卫生—妇产科学]