小檗碱通过Toll样受体4/核因子κB信号通路对小鼠病毒性心肌炎发挥保护作用  

Protective effect of berberine on viral myocarditis via Toll-like receptor 4/nuclear factor-κB signal pathway in mice

作　　者：李忠[1] 张培华[1] 

机构地区：[1]南阳理工学院张仲景国医学院,河南省南阳市473004

出　　处：《中国动脉硬化杂志》2017年第3期250-253,共4页Chinese Journal of Arteriosclerosis

基　　金：河南省科技攻关项目(152102310025)

摘　　要：目的探讨小檗碱对病毒性心肌炎小鼠的保护作用,并研究其对Toll样受体4(TLR4)、核因子κB(NF-κB)和肿瘤坏死因子α(TNF-α)的影响。方法将40只BALB/c小鼠按数字随机法均分为正常组、模型组、小檗碱低剂量组与高剂量组。除正常组外,其余组小鼠腹腔注射嗜心性柯萨奇病毒B3,建立病毒性心肌炎模型。小檗碱低剂量组与高剂量组分别腹腔注射小檗碱50 mg/kg与100 mg/kg。苏木素-伊红染色观察心肌组织病理学变化;用酶联免疫吸附测定试剂盒检测血清肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白(cTnT)和TNF-α水平;免疫印迹法和实时荧光定量PCR分别检测心肌组织中TLR4与NF-κB的蛋白表达和mRNA表达。结果与模型组相比,小檗碱治疗组心肌组织的炎性细胞浸润与心肌细胞坏死程度显著减轻,血清CK-MB、cTnT和TNF-α水平明显降低,心肌组织TLR4与NF-κB的蛋白表达和mRNA表达显著下调。结论小檗碱可通过调控TLR4/NF-κB信号通路,从而对病毒性心肌炎小鼠的心肌组织具有保护作用。Aim To investigate the protective effects of berberine on viral myocarditis in mice; To study the effects of berberine on Toll-like receptor 4（ TLR4）,nuclear factor-κB（ NF-κB） and tumor necrosis factor α（ TNF-α）. Methods According to random number table,40 BALB/c mice were divided into four groups： normal group,model group,low dose berberine treatment group and high dose berberin treatment group. Except the normal group,the mice of other groups were injected intraperitoneally with addicted concentric Coxsackie virus B3 to establish viral myocarditis model. Low dose berberine treatment group and high dose berberin treatment group were injected intraperitoneally with 50 mg/kg and 100 mg/kg respectively. The pathological changes of myocardial tissue were observed by HE staining. The serum levels of creatine kinase isoenzyme MB（ CK-MB）,cardiac troponin T（ cTnT） and TNF-α were determined by enzyme-linked immunosorbent assay kits. Western blot and real-time fluorescence quantitative PCR were used to detect the protein expression and mRNA expression of TLR4 and NF-κB in cardiac tissue. Results Compared with the model group,inflammatory cell infiltration and cell necrosis degree in myocardial tissues were significantly decreased,and the serum levels of CK-MB,cTnT and TNF-α were significantly reduced in the berberine treatment group. At the same time,the protein expression and mRNA expression of TLR4 and NF-κB were significantly down-regulated in myocardial tissue in the berberine treatment group. Conclusion Berberine can regulate the TLR4/NF-κB signal pathway,so as to protect the myocardial tissue of mice with viral myocarditis.

关 键 词：小檗碱 病毒性心肌炎 TOLL样受体4 核因子ΚB 肿瘤坏死因子Α 

分 类 号：R96[医药卫生—药理学]