利尿合剂抗炎镇痛及体外抑菌作用研究  

Anti-inflammatory and analgesic effects in vivo and bacteriostasis effect in vitro of diuretic mixture

作　　者：郭文贤[1] 戴卫波[1] 梅全喜[1] 李红念[1] 

机构地区：[1]广州中医药大学附属中山医院,广东中山528400

出　　处：《药物评价研究》2017年第2期201-205,共5页Drug Evaluation Research

基　　金：中山市科技计划项目(20132A166;2015B1049)

摘　　要：目的研究利尿合剂的抗炎镇痛及体外抑菌作用,为临床用药提供科学的理论依据。方法采用二甲苯致耳肿胀实验、冰醋酸致小鼠扭体反应实验、冰醋酸致小鼠腹腔毛细血管通透性增加实验及福尔马林致痛实验,观察利尿合剂高、中、低剂量组(以生药计18.0、9.0、4.5 g/kg)的抗炎镇痛作用;采用K-B纸片扩散法研究利尿合剂对金黄色葡萄球菌和大肠杆菌的体外抑菌作用。结果利尿合剂高剂量组可明显减轻小鼠耳廓肿胀度(P<0.01),高、中、低剂量明显减少冰醋酸致小鼠扭体反应次数(P<0.01),明显抑制冰醋酸致小鼠腹腔毛细血管通透性增加(P<0.05、0.01),显著降低福尔马林致痛实验的第I时相的疼痛强度(P<0.01),对第Ⅱ时相的疼痛强度没有明显影响;利尿合剂对金黄色葡萄球菌和大肠杆菌的抑菌率分别为95.04%、37.44%。结论利尿合剂具有较好的抗炎镇痛作用,对金黄色葡萄球菌和大肠杆菌具有体外抑菌作用。Objective To investigate the analgesic and anti-inflammatory effects of diuretic mixture and its bacteriostasis effect in vitro, and to provide scientific basis for clinical application. Methods Ear swelling test induced by xylene, twisting reaction test induced by acetic acid, capillary permeability increase in abdominal cavity of mice induced by acetic acid, and pain test induced by formalin were used to observe the analgesic and anti-inflammatory effects of diuretic mixture at high, middle and low doses（crude drug 18.0, 9.0, and 4.5 g/kg）. Bacteriostatic activities of diuretic mixture were tested by K-B paper disc diffusion method. Results Diuretic mixture alleviated ear edema in mouse model at high dose（P〈0.01）. Diuretic mixture at high, middle, and low dose could effectively decrease the twisting reaction（P〈0.01）, inhibit capillary permeability（P〈0.05 or 0.01）, and ease the ache degree of mice induced by formalin in the first phase（P〈0.01）, but there was no significant difference in the degree of pain intensity of phase II. The inhibitory rates of diuretic mixture on Staphylococcus aureus and Escherichia coli were 95.04% and 37.44%, respectively. Conclusion Diuretic mixture has significant effects on analgesia and anti-inflammation and against S. aureus and E. coli in vitro.

关 键 词：利尿合剂 抗炎镇痛 体外抑菌 耳肿胀 扭体反应 毛细血管通透性 

分 类 号：R965[医药卫生—药理学]