老年性痴呆-血瘀证病证结合树鼩模型的初步建立及三七总皂苷干预的评价  

Preliminary establishment of integration of Alzheimer's disease and blood stasis syndrome tree shrew model and evaluation of intervention of Panax notoginseng saponins

作　　者：陈奔[1] 覃梅春 黄金兰 吴登攀 郭尔楚 刘志萍[3] 许哲郝 郭茜茜[4] 钟振国[3] 

机构地区：[1]广西中医药大学广西中医药科学实验中心,广西南宁530001 [2]徐州医科大学药学院,江苏徐州221004 [3]广西中医药大学新药研究开发中心,广西南宁530001 [4]江西中医药大学,江西南昌330004

出　　处：《中国中药杂志》2017年第6期1175-1182,共8页China Journal of Chinese Materia Medica

基　　金：广西科学技术厅项目(桂科攻1347003-1)

摘　　要：初步建立老年性痴呆-血瘀证病证结合树鼩动物模型并以三七总皂苷进行药物干预,以该药物反证该病证结合模型的稳定性。以5种中医表征分析方法及凝血4项指标、血浆NO含量、血浆SOD含量评价各组树鼩模型的血瘀证水平;以免疫印迹试验(Western blot)检测各组树鼩大脑Aβ_(1-42)蛋白、淀粉样蛋白前体蛋白(APP)、磷酸化Tau蛋白(P-Tau)含量;HE染色观察各组树鼩大脑海马神经细胞形态,免疫组化法检测各组树鼩大脑海马胆碱乙酰基转移酶(ChAT)与突触素(SYP)含量。通过实验得出病证结合组树鼩血瘀证中医表征较AD组显著,给药组树鼩血瘀证表征较病证结合组减轻;与空白组相比,AD组树鼩大脑中Aβ_(1-42),APP及P-Tau含量降低,ChAT及SYP的平均阳性截面积/A降低,病证结合造模使这一降低加重,而三七总皂苷给药缓解了这一变化。说明AD-血瘀证病证结合树鼩模型具有稳定性。To establish the integration of Alzheimer＇s disease（AD） and blood stasis syndrome tree shrew model. Panax notoginseng saponins （PNS） was used to intervene the model to testify the stability of the model. The level of blood stasis of each group in the tree shrew model was evaluated by analyzing five traditional Chinese medicine（TCM） characterizations, four blood coagulation indexes, plasma nitric oxide （NO） level, plasma superoxide dismutase （SOD） level in each group. Hematoxylin and eosin（ HE ） staining was used to observe the morphological changes of brain hippocampal neuron cell of each group. Immunohistochemical staining was used to assay the ChAT and SYP levels in brain hippocampus of each group. The blood stasis characterization of the integration of disease and syndrome group was more obvious than the AD group, and that of the drug administration group was lower than that of the integration of disease and syndrome group. Aβ1-42, APP, P-Tau, ChAT and SYP level of AD group were lower than those in the blank group, which were further reduced in the model of integration of disease and syndrome. However, the administration of PNS relieved the reduction, indicating that the AD and blood stasis integration syndrome tree shrew model is stable.

关 键 词：老年性痴呆病 血瘀证 病证结合动物模型 树鼩 三七总皂苷 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]