不同慢性阻塞性肺疾病大鼠模型体内调节性T细胞变化  

Variation of regulatory T cell of different rat model with chronic obstructive pulmonary disease

作　　者：戴百章[1] 任朝凤[1] 郑勤玲[1] 杨艳霞[1] 李梅华[1] 

机构地区：[1]昆明市第一人民医院呼吸内科,云南昆明650000

出　　处：《临床肺科杂志》2017年第5期871-873,共3页Journal of Clinical Pulmonary Medicine

基　　金：云南省应用基础研究计划项目(No 2013ZF223)

摘　　要：目的通过对比不同大鼠模型体内调节性T细胞变化,探讨适于进行模拟研究慢阻肺患者体内调节性T细胞变化大鼠模型。方法大鼠随机分为3组,空白对照组,气道滴入脂多糖+烟熏建立慢阻肺模型组(脂多糖+烟熏组)、持续烟熏建立慢阻肺模型组(持续烟熏组),比较不同建模方案大鼠病理改变,研究不同建模方案大鼠体内T细胞变化。结果空白对照组与慢阻肺模型组平均内衬间隔之间结果有显著性差异;空白对照组与脂多糖+烟熏组平均肺泡数结果有显著性差异;持续烟熏组大鼠外周血Treg流式细胞检测计数低于空白对照组。结论持续烟熏组大鼠外周血Treg下降明显,炎症反应轻于脂多糖+烟熏组。持续烟熏大鼠可能更适于进行模拟研究慢阻肺患者体内调节性T细胞变化。Objective To compare the variation of regulatory T cell （Treg） of different rat model with chro- nic obstructive pulmonary disease （COPD） to choose appropriate COPD rat model studying change of Treg in vivo. Methods Rats were randomly divided into the control group, the lipopolysaccharide into airway group, and the continuous smoking group respectively. The pathology and the variation of regulatory T cell （Treg） of different rat model with COPD were compared. Results There was significant difference in pulmonary mean linear intercept （ MLI ） between the control group and the COPD group. Mean alveolus number was significantly different between the control group and the lipopolysaccharide and smoking group. Treg of peripheral blood counting by flow cytometry in the smoking group was lower than in the control group. Conclusion There is lower Treg of peripheral blood in the smoking group and lighter inflammation comparing with the lipopelysaccharide and smoking group. Smoking rat may be more suitable for stimulate studying the variation of regulatory T cell in patients with COPD.

关 键 词：慢性阻塞性 大鼠 调节性T细胞 

分 类 号：R563.9[医药卫生—呼吸系统] R-332[医药卫生—内科学]