应用荧光定量PCR对PCV2在不同类型猪群血液中感染情况的调查  

Investigation of PCV2 Infection in Various Pig's Blood by Fluorescent Quantitative PCR Assay

作　　者：张福良[1] 王国栋[1] 靳国旺[1] 

机构地区：[1]安阳工学院生物与食品工程学院,河南安阳455000

出　　处：《安阳工学院学报》2017年第2期96-100,共5页Journal of Anyang Institute of Technology

基　　金：安阳工学院校科研基金项目"生猪重大疫病预警系统的建立与示范应用";安阳市科技计划项目(20151230151743137);安阳市科技计划项目(20161219193534546)

摘　　要：应用荧光定量PCR对某规模化养猪场340头份不同类型猪群猪只血液中PCV2感染的情况进行了调查。结果表明,所有被检测的猪群均存在PCV2感染,不同类型猪群的感染率为100%,全场猪的平均感染率为76.8%(261/340)。其中,生长肥育猪群PCV2感染率最高为90%,而种公猪群感染率最低为30%。在病毒荷载量方面,整个猪群的血液中病毒荷载量处于103~104copies/μL之间。The PCV2 infection was determined by fluorescent quantitative PCR assay in 340 serum samples col-lected from various pig groups in a pig farms in Guangdong province. The result showed that the PCV2 inflectionwas consisted in all pig groups, and the total inflection ratio was 76.8 %（ 261/340）. The inflection ratio in growinggroups was the highest（90%）,while which was the lowest in pig＇s blood in boars groups（30%）.Meanwhile, the re-sult indicated that the content of PCV2 in various pig＇s blood was from 103 to 104copies/μL.

关 键 词：猪圆环病毒2型 荧光定量PCR 感染率 

分 类 号：S855.3[农业科学—临床兽医学]