胡杨组织培养叶片及插穗毛状根发生  

Rooting Induced on in vitro Leaves and Fresh Cuttings of Populus euphratica

作　　者：王大鹏[1] 唐嘉泽 邵明成[1] 张文彪 王华芳[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京市延庆区第一职业学校,北京102100

出　　处：《植物学报》2017年第2期210-217,共8页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31370598;No.J1103516)

摘　　要：胡杨(Populus euphratica)是唯一分布在干旱沙漠地区的高大乔木,根蘖能力强,但自然条件下插穗难以生根,扦插繁殖困难。该文研究发根农杆菌菌株ATCC11325诱导胡杨发根,菌液浓度为OD_(600)=0.4,添加100 mg·L^(–1)乙酰丁香酮,叶片切段划伤预培养后侵染10分钟,培养20天,在叶片创伤部位诱导发生无向性的健壮毛状根;浸染肉质根插穗,须根发生和植株成活率从20%提高到60%,且地上部分分枝多,生长茂盛。该发根农杆菌菌株显著促进胡杨插穗生根成活。Populus euphratica is the unique natural distribution arbor tree growing in the dry desert environment, with root suckering, but cuttings of roots are difficult to grow. This study explored the new ways of rooting induced by Agrobacterium rhizogenes. The strain ATCC11325 can induce hairy roots with no-tropism on in vitro leaf sections. The leaves were cut, scarred, co-cultivated, soaked for 10 min in the microbial agent at OD_（600）=0.4 with AS 100 mg·L^（–1） added and cultured on tissue culture media for 20 days. Hairy rooting occurred on fresh cuttings soaked with the A. rhizogenes strain solution. The rooting and survival rate reached 60%, and aboveground parts developed more branches and showed vigorous growth. The A. rhizogenes strain promoted rooting of poplar tree fresh cuttings.

关 键 词：发根农杆菌 毛状根 ROLB基因 生根 胡杨 

分 类 号：S792.11[农业科学—林木遗传育种]