miRNA单核苷酸多态性与宫颈癌的相关性研究  

Association between single nucleotide polymorphisms of microRNA and cervical cancer risk

作　　者：王春桃[1] 何曙芝[1] 徐敏 张艳秋[3] 隋静[3] 申娴[3] 梁戈玉[3] 

机构地区：[1]盐城卫生职业技术学院护理学院,江苏盐城224001 [2]盐城市第一人民医院,江苏盐城224005 [3]环境医学工程教育部重点实验室/东南大学公共卫生学院,江苏南京210009

出　　处：《现代预防医学》2017年第7期1220-1224,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(81472939;81673132);江苏省高校哲学社会科学研究项目(2016SJB880119);盐城市医学科技发展计划项目(YK2016055;YK2013060)

摘　　要：目的探讨miR-27a、miR-34a/c和miR-107单核苷酸多态性与宫颈癌的相关性。方法收集104例宫颈癌及186例对照,采用多重PCR技术对miR-27a,miR-34a/c和miR-107单核苷酸多态性进行分析,卡方检验分析各基因型与宫颈癌之间的相关性。结果在病例组和对照组中,miR-27a rs895819 TT、CT和CC基因型的分布为52.9%、44.1%、2.9%和59.8%、33.7%、6.5%,miR-34b/c rs4938723 TT、CT和CC基因型的分布为51.9%、35.6%、12.5%和47.3%、44.1%、8.6%,miR-107 rs2296616 TT、CT和CC基因型的分布为80.8%、19.2%、0%和81.2%、17.7%、1.1%,各位点基因型频数分布差别均无统计学意义(P>0.05)。进一步分层分析发现,不同年龄的各基因型分布在对照组和病例组中差异也均无统计学意义(P>0.05)。结论 miR-27a rs895819、miR-34b/c rs4938723和miR-107 rs2296616基因多态性可能与宫颈癌发生无关。Objective To investigate the relationship between gene polymorphism of miR -27a, miR - 34a/c and miR - 107 and cervical cancer risk. Methods A case - control study was carried out including 104 patients with cervical cancer and 186 controls. The gene polymorphism of miR -27a, miR -34a/c and miR - 107 in patients and controls were detected by multiple PCR method. The association between the genotypes and cervical cancer was analyzed by Chi - square test. Results The genotypes ofmiR-27a rs895819 were 52.9% for TT,44.1% for CT,2.9% for CC in eases and 59.8% for Tr,33.7% for CT,6.5% for CC in controls. The genotypes of miR -34b/c rs4938723 were 51.9% for TF,35.6% for CT, 12.5% for CC in cases and 47.3% for TT,44.1% for CT,8.6% for CC in controls. The genotypes of miR - 107 rs2296616 were 80.8% for TT, 19.2% for CT,0% for C C in eases and 81.2% for TF, 17.7% for CT, 1.1% for CC in controls. The genotype distribution of miR-27a rs895819,miR- 34b/c rs4938723 and miR- 107 rs2296616 polymorphism in the cases and the controls had no significantly statistical difference（ P 〉 0.05 ）. Further age -stratified analysis showed that these three polymorphism genotype frequencies distribution had no statistical significance in the eases and the controls （ P 〉 0.05 ）. Conclusion There is no association between gene polymorphism of miR -27a rs895819 ,miR -34b/c rs4938723 and miR - 107 rs2296616 and cervical cancer.

关 键 词：miR-27a miR-34b/c miR-107 基因多态性 宫颈癌 

分 类 号：R17[医药卫生—妇幼卫生保健]