IFN-λs及其JAK-STAT信号通路中相关分子在MSMHIV-1感染病人的变化规律  

Expression patterns of IFN-λs and related molecules of JAK-STAT signaling pathway of HIV-1 infected individuals in MSM

作　　者：赵国宪[1,2] 刘利锋[1,2] 李威[1,2] 粟斌[1,2] 张彤[1,2] 吴昊[1,2] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069 [2]首都医科大学北京佑安医院感染中心艾滋病研究北京市重点实验室,北京100069

出　　处：《中国艾滋病性病》2017年第3期194-198,共5页Chinese Journal of Aids & STD

基　　金：艾滋病研究北京市重点实验室(BZ0089);北京市医院管理局临床医学发展专项经费(ZY201401);北京市科技计划资助(D141100000314002;314005;416003);国家自然科学基金(81571973)~~

摘　　要：目的研究男男性行为者(MSM)艾滋病(AIDS)病人急性期、慢性期外周血单核细胞(PBMC)中的干扰素(IFN)及其JAK-STAT信号传导通路中相关分子,以期发现IFN-λs、IFN-λR及相关基因、蛋白在HIV不同感染时期的变化规律。方法收集PBMC标本,用实时荧光定量聚合酶链反应(RT-PCR)及流式细胞术方法分析IFN-α/βR2、IFN-γReceptor 1、IFN-λR1、STAT1、STAT2、STAT3、STAT4、STAT5a、STAT5b、STAT6、IRF9、Mx1、OAS1信使核糖核酸(mRNA)及蛋白的表达。结果对20例急性HIV-1感染(AHI)病人、24例慢性HIV-1感染(CHI)病人、22例健康对照(HC)的PBMC标本进行分析,RT-PCR结果显示,在急性HIV-1感染患者中,IFN-αR2、IFN-γReceptor1、IFN-λR1、STAT1、MX1的mRNA水平高于健康对照组(分别为6.03、9.89、8.20、7.03、5.89倍)(P<0.01)。IFN-λR1的mRNA水平与CD4+T淋巴细胞计数呈负相关(R=-0.403,P=0.009),与病毒载量呈正相关(R=0.43,P=0.005)。流式细胞术结果显示,在急性HIV-1感染患者中,IFN-αR2、IFN-γReceptor1、IFN-λR1、STAT1的蛋白水平高于健康对照组(P<0.01)。结论急性HIV-1感染可以提高IFN-λR1及相关基因、蛋白的表达,并且与疾病进展相关。Objective To study the interferon and related molecules of JAK-STAT signaling pathway in PBMC of the acute and chronic AIDS patients infected via homosexual transmission, and to find out the expression pat- terns of IFN-λs, IFN-λR and related genes and proteins in different HIV-1 infection period. Methods The PBMC specimens were collected. The mRNA and protein levels of IFN-alpha/beta R2, IFN gamma Receptor 1, IFN-lamb- da R1, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, STAT6, IRF9, Mxl, OAS1 were measured by RT-PCR and flow eytometry. Results 20 patients with acute HIV-1 infection （AHI）, 24 patients with chronic HIV-1 infection （CHI） and 22 healthy controls （HC） were analyzed. The results of RT-PCR showed that the ex- pression of IFN-alpha receptor, IFN-gamma receptor, IFN-lambda receptor, STAT1 and Mxl mRNA levels in acute HIV infected patients were higher （6.03, 9.89, 8.20, 7.03, 5.89 fold, respectively） than those in healthy controls （P〈0.01）. The mRNA levels of IFN-lambda R1 negatively correlated with CD4^＋ T cells （R= -0. 403, P=0. 009） and positively correlated with viral load （R= 0. 43, P〈0. 005）. The results of flow cytometry showed that the expression of IFN-alpha receptor, IFN-gamma re- ceptor, IFN-lambda receptor and STAT 1 protein levels were higher than those in healthy controls （P〈0. 01）. Conclusion The acute HIV-1 infection can improve the expression of IFN-lambda R1 and related genes and proteins and correlated with disease progression.

关 键 词：艾滋病病毒1型 IFN-λ 信号通路 疾病进展 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]