miRNA-146a在脓毒症引起的急性肺损伤中的作用机制  

Mechanism of action of miRNA-146a in sepsis-induced acute lung injury

作　　者：孟建斌[1] 孙丽萍[1] 瓦永禄[1] 刘丽莉[1] 姚茜[1] 

机构地区：[1]青海红十字医院重症医学科,西宁810000

出　　处：《解放军医学杂志》2017年第3期207-210,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨miRNA-146a在脓毒症(sepsis)引起的急性肺损伤(ALI)中的表达及其对炎症的干预作用。方法选取脓毒症引起的ALI患者及健康志愿者,采用q RT-PCR检测其外周血miRNA-146a的相对表达量。建立脓毒症引起的ALI小鼠模型,注入miRNA-146a模拟物,miRNA-146a对照剂和miRNA-146a抑制剂进行干预。24h后测定小鼠肺组织miRNA-146a及外周血肿瘤坏死因子α(TNF-α)、白介素(IL)-1β、环氧化酶2(COX-2)等炎性因子的m RNA表达水平。结果脓毒症引起的ALI患者外周血miRNA-146a表达量明显高于健康对照组(P<0.05)。动物实验发现,与miRNA-146对照组比较,miRNA-146a组小鼠TNF-α、IL-1β和COX-2表达量均明显降低,而miRNA-146a抑制组小鼠TNF-α、IL-1β和COX-2表达量明显上升(P<0.05)。miRNA-146a+COX-2共同抑制组肺部TNF-α、IL-1β表达量明显低于miRNA-146a单独抑制组(P<0.05)。结论 miRNA-146a可通过靶向COX-2有效降低脓毒症引起的炎性反应。Object To study the expression of miRNA-146 a in sepsis-induced acute lung injury（ALI） patients and its effect on the inflammation in mouse models. Methods miRNA-146 a expression in peripheral blood was determined in sepsisinduced ALI patients and healthy volunteers by q RT-PCR. Sepsis-induced ALI mouse model were reproduced by LPS treatment and miRNA-146 a mimic, miRNA-146 a NC and miRNA-146 a inhibitors were injected through trachea. The expressions of miRNA-146 a, TNF-α, IL-1β and COX-2 m RNA were determined by q RT-PCR 24 h after the intervention. Results The miRNA-146 a expression in peripheral blood significantly increased in severe sepsis with ALI patients, compared with the control subjects. For mouse model, the expressions of TNF-α, IL-1β and COX-2 m RNA significantly decreased in miRNA-146 a group, while increased in miRNA-146 a inhibitor group compared with miRNA-146 a NC group（P〈0.05）. The TNF-α and IL-1β expressions significantly decreased in miRNA-146 a inhibitor＋COX-2 inhibitor group compared with miRNA-146 a inhibitor group（P〈0.05）. Conclusion Mi R-146 a treatment can effectively alleviate the lung inflammation in sepsis-induced ALI mice.

关 键 词：miRNA-146a 脓毒症 急性肺损伤 

分 类 号：R563.8[医药卫生—呼吸系统]