过路黄抗肿瘤活性成分研究  

Anti-tumor components from Lysimachia christinae Hance

作　　者：崔慧敏[1] 董俊兴[1] 刘士军[1] 李彬[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2017年第3期213-217,共5页Military Medical Sciences

摘　　要：目的寻找过路黄(Lysimachia christinae Hance)抗肿瘤活性成分。方法采用AB-8大孔吸附树脂色谱、硅胶层析柱色谱、Sephadex LH-20凝胶柱色谱、ODS柱色谱和制备HPLC等技术方法进行化学成分分离,根据理化性质和波谱数据鉴定化合物结构。MTT法检测化合物体外抗肿瘤细胞生长活性。结果从过路黄干燥全草70%乙醇提取物中分离得到11个化合物,分别鉴定为:3,3'-二甲氧基-6,6'-双[(Z)-十五烷-10-烯-1-基)]-(1,1'-双环己烷)-3,3',6,6'-四烯-2,2',5,5'-四酮(1),大黄素甲醚(2),茜草色素(3),9,16-二羰基-10,12,14-三烯十八碳酸(4),西克拉敏A糖苷A-3-O-{β-D-吡喃木糖基-(1→2)-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→4)]-α-L-吡喃阿拉伯糖苷}(5),原报春花素A-3-O{β-D-吡喃木糖基-(1→2)-β-D-吡喃葡萄糖基-(1→4)-[β-D-吡喃葡萄糖基-(1→2)]-α-L-吡喃阿拉伯糖苷}(6),槲皮素-3-O-吡喃葡萄糖苷(7),异鼠李素-3-O-β-D-吡喃半乳糖苷(8),3',4',5',5,7-五羟基黄酮(9),芦丁(10),槲皮素(11)。化合物5对人宫颈癌细胞株He La、人骨肉瘤细胞株U20S、人肺腺癌细胞株PC-9、人结肠癌细胞株CT-26的生长均有一定的抑制作用,其IC50值分别为2.29、1.22、1.43、1.29μmol/L。结论化合物1为新化合物,化合物2~4为首次从该属植物中分离得到。化合物5在体外具有显著抗肿瘤细胞生长活性。Objective To isolate and identify anti-tumor constituents from Lysimachia christinae Hance. Methods The compounds were isolated and purified by chromategraphy on AB-8 macroporous resin,silica gel,Sephadex LH-20,ODS and preparative HPLC. Their structures were elucidated using chemical and spectroscopic methods. All the compounds obtained were evaluated on their growth inhibitory effects in vitro using the MTT method. Results Eleven compounds were isolated from the whole plant of L. christinae Hance and were identified as 3,3＇-dimethoxy-6,6＇-di[（ Z）-pentadec-10-en-1-yl]-[1,1＇-bi（ cyclohexane） ]-3,3＇,6,6＇-tetraene-2,2＇,5,5＇-tetraone（ 1）,physcion（ 2）,munjistin（ 3）,9,16-dioxooctadec-10,12,14-trienoic acid（ 4）,cyclamiretin A-3-O-{ β-D-xylopyranosyl-（ 1→2）-β-D-glucopyranosyl-（ 1 →2）-[β-D-glucopyranosyl-（ 1 → 4） ]-α-L-arabinopyranoside（ 5）,lysikoianoside（ 6）,quercetin-3-O-glucopyranoside（ 7）,isorhamnetin3-O-β-D-galactopyranoside（ 8）,3＇,4＇,5＇,5,7-pentahydroxyflavone（ 9）,rutin（ 10）,and quercetin（ 11）. The screening results of antitumor activity in vitro showed that IC50 of compound 5 for human cervical carcinoma cells He La,human osteosarcoma cells U20 S,human lung adenocarcinoma cells PC-9,and human colorectal carcinoma cells CT-26 was2. 29,1. 22,1. 43,and 1. 29 μmol/L respectively. Conclusion Compound 1 is a new compound and compounds 2-4are isolated from the plants of Lysimachia for the first time. Compound 5 shows obvious inhibitory effects on tumor cell growth in vitro.

关 键 词：金钱草(过路黄) 化学成分 抗肿瘤药 植物 新化合物 色谱法 高压液相 

分 类 号：R284[医药卫生—中药学]