星点设计-效应面法优化莪术油中吉马酮的提取工艺  

Optimization of Extraction Process of Germacrone from Zedoary Turmeric Oil by Central Composite Design and Response Surface Methodology

作　　者：吴杰[1] 冯钰[1] 陈伟强[2] 杨梦蝶 古金华[1] 叶连宝[1] 

机构地区：[1]广东药科大学药科学院 [2]广东药科大学基础学院,广州510006

出　　处：《天然产物研究与开发》2017年第3期497-502,共6页Natural Product Research and Development

基　　金：广东省自然科学基金(2015A030313586);广东省科技计划(2015-110)

摘　　要：利用星点设计-效应面优化法优化莪术油中吉马酮的提取工艺,并对其进行含量测定。首先采用水蒸气蒸馏法提取莪术油,选取吉马酮含量为因变量,在单因素试验基础上,选取浸泡时间、提取时间、液料比为自变量,采用星点设计考察自变量对提取工艺的影响,通过对不同因素各水平的多元线性回归及二项式拟合,运用效应面法优选提取工艺并进行预测分析;运用HPLC法以Kromasil C_(18)柱(250×4.6 mm,5μm);甲醇-水(80∶20)为流动相;1.0 mL/min为流速,在220 nm处测定吉马酮的含量。所建立的含量测定方法线性关系、精密度、重复性、稳定性、加样回收率试验结果良好,最佳提取工艺条件为浸泡时间2.36 h,提取时间8.19 h,加水量9.98倍;依最佳条件提取的吉马酮平均得率8.13%,与理论预测值的偏差较小。星点设计-效应面优化法优选的提取工艺简便合理、预测性良好,为吉马酮的资源开发提供参考。Zedoary turmeric oil from crude extract of Rhizoma Cttrcuma has been used as an anti-cancer agent in Tradi- tional Chinese Medicine. Germacrone is a natural compound isolated from zedoary turmeric oil. In this study, we opti- mized the extraction process of germacrone from zedoary turmeric oil by central composite design and response surface methodology. The process was carried out with the content of germacrone as dependent variable, soaking time, extraction time and the amount of water as independent variables which was based on single factor tests, and each level of inde- pendent variables was made by multiple linear regression and binomial fitting. Response surface methodology was used to optimized extraction process conditions and carry on predictive analysis. The HPLC method was used to test the determi- nation of germacrone. The results showed that average content of germacrone was 8.13% with a small deviation com- pared with the predicted value when optimized extraction conditions were applied with soaking time of 2.36 h, extraction time of 8.19 h and 9.98 times water. This optimized extraction process was simple and reasonable with a good predicta- bility and can provide reference for extraction of germacrone.

关 键 词：莪术油 吉马酮 星点设计-效应面法 提取工艺 含量测定 

分 类 号：R28[医药卫生—中药学]