GLP-1类似物对2型糖尿病SD大鼠糖、脂代谢和血管超微结构的影响  

Effects of GLP-1 analogues on glucose,lipid metabolism and vessel ultrastructure in type 2 diabetic SD rats

作　　者：隋春华[1] 翟华玲[1] 郭郁郁[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院内分泌科,上海200011

出　　处：《中国医药导报》2017年第7期4-7,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81300653)

摘　　要：目的研究GLP-1类似物对2型糖尿病SD大鼠糖、脂代谢和血管超微结构的影响。方法采用随机数字表法将28只SD大鼠随机分成四组:正常对照组(C组,n=6),正常对照+Exenatide干预组(C+E组,N=6),2型糖尿病组(D组,n=8),2型糖尿病+Exenatide干预组(D+E组,n=8)。观察四组大鼠体重、胰岛素敏感指数、血糖、胰岛素、血脂、胰岛素抵抗指数,并电子显微镜观察血管超微结构的改变。结果 Exenatide治疗前后各组大鼠体重、空腹血糖、空腹胰岛素、血脂谱、胰岛素抵抗指数、胰岛素敏感指数差异均有统计学意义(P<0.05)。电子显微镜显示,D组大鼠血管内皮细胞欠完整,内皮下见胶原纤维增生,线粒体肿胀;D+E组大鼠血管内皮细胞较完整,个别线粒体肿胀。结论 GLP-1类似物能改善糖尿病大鼠血管超微结构,从而改善其功能。Objective To study the effects of GLP-1 analogues on glucose, lipid metabolism and vessel ultra-structure in type 2 diabetic SD rats. Methods Twenty-eight SD rats were randomly divided into four groups by the method of random number table： normal rats （group C, n=6）, normal rats treated with Exenatide （group C＋E, n=6）, diabetic rats （group D, n=8）, diabetic rats treated with Exenatide （group D＋E, n=8）. The body weight, fasting glucose, fasting insulin, insulin resistance index, insulin sensitivity index, lipid profile of the four groups were observed, while the uhrastruetural changes of the vessel before and after treatment by GLP-1 analogues of the four groups using electron microscopy were observed. Results Bodyweight, fasting glucose, fasting insulin, insulin resistance index, insulin sensitivity index, lipid profiles of rats in each group were significantly improved and had statistically significant differents （P 〈 0.05） by treatment with GLP-I analogues. Electron microscopy showed that, in group D, vascular endothelial cells were not in- tact, and the proliferation of collagen fibers was seen, mitochondria swelled. In group D＋E, vascular endothelial cells were still intact, individual mitochondrial swelled. Conclusion GLP-1 analogues can improve vessel ultrastructure in diabetic rats, thereby improving its functionality.

关 键 词：GLP-1类似物 糖尿病 血管超微结构 

分 类 号：R587.1[医药卫生—内分泌]