2株枯草芽孢杆菌对太子参4种病原真菌的抑菌效果研究  

Inhibitory effect of two Bacillus subtilis strains on four kinds of pathogenic fungi in Radix Pseudostellariae

作　　者：张国辉[1] 范成明[2] 佀胜利[1] 

机构地区：[1]凯里学院环境与生命科学分院,贵州凯里556011 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《中国植保导刊》2017年第3期18-23,共6页China Plant Protection

基　　金：贵州省普通高等学校创新团队项目(黔教合人才团队字[2014]48号);贵州省科技厅联合基金课题(黔科合J字LKK[2013]11号);凯里学院2014年"精品资源共享课"微生物学(JZGX201404);贵州省教育质量提升工程项目(黔教合KY字[2014]228号)

摘　　要：采用枯草芽孢杆菌(Bacillus subtilis)菌株GH18和GH19对贵州省黔东南州太子参(Pseudostellaria heterophylla)的病原真菌互隔交链孢霉菌(Alternaria alternata)、博宁刺盘孢(Colletotrichum boninense)、胶孢炭疽菌(Colletotrichum gloeosporioides)和立枯丝核菌(Rhizoctonia solani)进行了抑菌效果研究。结果表明,菌株GH18和GH19对4种病原真菌都有抑菌效果,菌株GH18无细胞滤液对互隔交链孢菌孢子的抑菌效果优于GH19。菌株GH18高温杀死菌体发酵液在浓度为2.0×10~8 cfu/L和2.0×10~7 cfu/L分别处理,对互隔交链孢霉菌、博宁刺盘孢抑菌率相近。处理3d的抑菌效果优于4 d;其发酵液的抑菌率高于无细胞滤液。菌株GH18高温杀死菌体发酵液在浓度为2.0×10~8cfu/L对互隔交链孢霉菌、博宁刺盘孢抑菌效果较好,胶孢炭疽菌效果次之。因立枯丝核菌气生菌丝生长旺盛,抑菌率较低,故不适合采用生防菌GH18和GH19进行生防。Anti-bacterial effect of Bacillus su btilis strain GH 18 and GH19 on pathogenic fungi,Alternaria arborescens,Colletotrichum boninense, Colletotrichum gloeosporioides and Rhizoctonia solani in Pseudostellaria heterophylla were studied in Qiandongnan prefecture of Guizhou province. The results showed that antibacterial rate against pathogenic fungi was similar of GH18 bacteria sterilizing solution at a concentration of 2.0 ×10^8 cfu/L and 2.0 ×10^7 cfu/L, the antibacterial effect of 3 d was better than that of 4 d, and the antibacterial effect of GH18 was better than that of GH19. Inhibition rate of fermentation broth by high temperature was higher than that of cell-free filtrate. Inhibitory effect of fermentation liquid of GH18 at high temperature with spore concentration of 2.0 ×10^8cfu/L on A. arborescens, C. boninense was better than that on C. gloeosporioides. While the inhibitory rate of R. solani was low, because of its aerial mycelium growth, which indicated that it was not a suitable bio-control target using GH18 and GH19.

关 键 词：太子参 生防菌 互隔交链孢霉菌 博宁刺盘孢 胶孢炭疽菌 立枯丝核菌 抑菌率 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治] S482.292[农业科学—植物保护]