替米沙坦对胰岛素抵抗大鼠PPAR-γ蛋白表达的影响  

Effects of telmisartan on the expression of PPAR-γ protein in OLETF rats with insulin resistance

作　　者：任梦萌 王伟占 方涛[3] 田凤石[2] 

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津市第四中心医院心内科,天津300140 [3]天津市第四中心医院中心实验室,天津300140

出　　处：《天津医科大学学报》2017年第2期124-127,共4页Journal of Tianjin Medical University

基　　金：天津市应用基础与前沿技术研究计划(青年项目)基金资助(15JCQNJC13100)

摘　　要：目的:观察替米沙坦对胰岛素抵抗(IR)大鼠肝脏、肾周脂肪和骨骼肌组织PPAR-γ蛋白表达的影响。方法:雄性OLETF大鼠40只,高脂饲养20周建立IR模型后,随机分为4组(n=10只),即高脂对照组(O)、吡格列酮组(P)、低剂量替米沙坦组(VT)和替米沙坦组(T),进行药物干预。O组单纯高脂喂养,P组给予高脂加吡格列酮,VT组给予高脂加低剂量替米沙坦,T组给予高脂加替米沙坦。LETO大鼠12只为NC(正常对照)组,单纯予标准饲料。干预20周后测定各组大鼠FBG、FINS、HOMAIR、ISI、TC、TG、HDL-C、LDL-C水平。Western blot测定各组织PPAR-γ蛋白表达。结果:40只OLETF大鼠均出现IR,造模成功。干预20周后,O组FBG、FINS、HOMAIR、TC、TG和LDL-C水平均高于P、VT和T组,ISI、HDL-C均低于P、VT和T组,根据Western blot结果可看出P、VT、T组所取各组织PPAR-γ蛋白表达均高于O组(均P<0.05);VT组FBG、FINS、HOMAIR、TC、TG、和LDL-C水平均高于T组,ISI、HDL-C水平低于T组,且T组比VT组各组织PPAR-γ蛋白表达较高(均P<0.05)。结论:替米沙坦可通过上调肝脏、肾周脂肪及骨骼肌组织PPAR-γ蛋白的表达来改善IR,且改善程度可能与替米沙坦剂量有关。Objective： To observe the effects of telmisartan on the expression of PPAR-γ protein in rats tissues including liver, muscle and perirenal fat with IR. Methods： Forty male OLETF rats were fed with high-fat diet for 20 weeks to establish the IR model, then randomly assigned into four groups： the high-fat diet set（O） were given pure high-fat diet, the Pioglitazone group （P） were fed with high-fat diet and Pioglitazone, the low dose Telmisartan group （VT） were fed with high-fat diet and low dose Telmisartan, and rats in the Telmisartan group （T） were fed with high-fat diet and Telmisartan. Twelve LETO rats raised by standard feeding served as normal control group （NC）. Following 6 weeks-drug intervention, the plasma levels of FBG, FINS, HOMA-IR, ISI, TC, TG, HDL-C, LDL-C were detected in four groups, and the expression level of PPAR-γ protein was measured using Western blot in each tissues . Results： Insulin resistance was observed in all 40 rats and buildings were established. Compared with group P, VT and T, the FBG, FINS, HOMA-IR, TC, TG, and LDL-C in O group were significantly increased. ISI and HDL-C were decreased （P 〈0.05）. The expressions of PPAR-γ protein in group P, VT and T were higher than group O. And the group T was lower than VT group in biochemical index results except for ISI and HDL-C; and the expression of PPARγ protein was higher. Conclusion： Telmisartan can significantly improve IR through increasing the expression of PPAR-γ protein in liver, perirenal fat and skeletal muscle tissues; and the improvement degree may be associated with telmisartan dose.

关 键 词：胰岛素抵抗 替米沙坦 吡格列酮 过氧化物酶体增殖物激活受体Γ 大鼠 

分 类 号：R58[医药卫生—内分泌]