柱前衍生HPLC法测定生、炒决明子中七种单糖的含量  

Determination of Seven Monosaccharides in Raw and Fried Cassiae Semen by Precolumn Derivatization HPLC

作　　者：万敏[1] 米宝丽[1] 张振秋[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中华中医药学刊》2017年第4期834-837,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家中医药行业科研专项项目(20110700703)

摘　　要：目的:建立决明子中葡萄糖、半乳糖、木糖、甘露糖、阿拉伯糖、葡萄糖醛酸、岩藻糖等7个单糖的HPLC测定方法,并比较其在生、炒决明子中的含量差别。方法:采用Kromasil C18色谱柱(150 mm×4.6 mm,5μm),流速为1.0 m L·min^(-1),检测波长为245 nm。流动相为乙腈(A)-水(B)、乙腈(A)-0.1%磷酸水(B),梯度洗脱,分别测定葡萄糖、半乳糖、木糖的含量与甘露糖、阿拉伯糖、葡萄糖醛酸、岩藻糖的含量。结果:生、炒决明子中葡萄糖、半乳糖、木糖、甘露糖、阿拉伯糖、葡萄糖醛酸、岩藻糖线性范围分别为0.028 8~1.15(r=0.999 6)、0.022 0~0.882(r=0.999 4)、0.025 8~1.03(r=0.999 5)、0.051 4~2.05(r=0.999 5)、0.010 6~0.426(r=0.999 3)、0.031 4~1.26(r=0.999 4)、0.047 8~1.19(r=0.999 3);加样回收率(n=5)在98.1%~99.3%,RSD在0.9%~1.7%。结论:炒决明子中单糖含量显著高于生决明子。Objective： To establish an HPLC method for determination of glucose,galactose,xylose,mannose,arabinose,glucuronic acid and fucose in Cassiae Semenand make a comparison between the crude and stirfrying Cassiae Semen. Methods： Chromatographic conditions concluded Kromasil C18（ 150 mm × 4. 6 mm,5 μm） column at the flow rate of1. 0 m L/min. The detection wavelength was set at 245 nm. The mobile phaseⅠ was acetonitrile-0. 1% phosphoric acid in gradient elution mode for determination of glucose,galactose and xylose. The mobile phase Ⅱ was acetonitrile-water in gradient elution mode for determination of mannose,arabinose,glucuronic acid and fucose. Results： Glucose,galactose,xylose,mannose,arabinose,glucuronic acid and fucose had good linearity in the ranges of 0. 028 8 - 1. 15（ r =0. 999 6）,0. 022 0 - 0. 882（ r = 0. 999 4）,0. 025 8 - 1. 03（ r = 0. 999 5）,0. 051 4 - 2. 05（ r = 0. 999 5）,0. 010 6 - 0.426（ r = 0. 999 3）,0. 031 4 - 1. 26（ r = 0. 999 4）,0. 047 8 - 1. 19（ r = 0. 999 3）,respectively. The average recoveries of the seven constituents were 98. 1% - 99. 3% and RSDs were 0. 9%-1. 7%. Conclusion： The contents of seven monosaccharides in processed Cassiae Semen decreased.

关 键 词：决明子 单糖 炮制 柱前衍生 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]