养正消积胶囊对胃癌组织Foxp3基因和相关细胞因子表达的影响  

Intervention of Expression of Foxp3 in Gastric Carcinoma Inssure and Related Cytokines after Using Yangzheng Xiaoji Capsule

作　　者：钱祥[1] 张爱琴[1] 周群琴 吴涛 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022 [2]绍兴市柯桥区中医院,浙江绍兴312030 [3]嘉善县人民医院,浙江嘉善314100

出　　处：《中华中医药学刊》2017年第4期904-907,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81402429);浙江省中医药科技计划项目(2014ZB020)

摘　　要：目的:探讨养正消积胶囊对胃癌组织Foxp3mRNA、scurfin蛋白及血清Il-10、TGF-β1表达的影响。方法:用皮下注射法造模成功后,分为模型组、养正消积胶囊组、5FU组、联合组,另设正常组,每组10只。分别用免疫荧光定量、蛋白质印迹、酶联免疫吸附法进行Foxp3mRNA、scurfin蛋白、IL-10、TGF-β1的检测。结果:养正消积胶囊组、联合组Foxp3mRNA、scurfin蛋白、IL-10、TGF-β1的表达较模型组明显降低,有统计学意义(P<0.05)。结论:养正消积胶囊能抑制Foxp3mRNA、scurfin蛋白以及IL-10和TGF-β1的表达,逆转肿瘤免疫逃逸,起到抗癌作用。Objective： To explore the intervention of expression of Foxp3 mRNA,scurfin protein in gastric carcinoma tissure and IL-10 and TGF-β1 in serum after using Yangzheng Xiaoji Capsule. Methods： MFC tumor bearing 615 mice models were established by inoculated subcutaneously way and randomly divided into 4 groups： model group,Yangzheng Xiaoji Capsule group,5FU group,comprehensive treatment group. Each group had 10 mice and normal group also had10 mice. Use RT-PCR and Western Blot to detect the expression levels of Foxp3 mRNA and scurfin protein of transplanted tumor tissue. Use ELISA to detect the expression levels of IL-10 and TGF-β1 in serum. Results： The expressions of Foxp3 mRNA,scurfin protein,IL-10 and TGF-β1 of 615 mice in model group,Yangzheng Xiaoji Capsule group and comprehensive treatment group were lower than those in model group（ P〈0. 05）. Conclusions： Yangzheng Xiaoji Capsule inhibits expressions of Foxp3 mRNA,surfin protein,IL-10 and TGF-β1 and reverse tumor immune escape for treating cancer.

关 键 词：养正消积胶囊 胃癌 Foxp3mRNA scurfin IL-10 TGF-Β1 

分 类 号：R285.5[医药卫生—中药学]