人参茎叶皂苷的Fusarium sacchari转化产物成分研究  

Chemical constituents in transformation product of Fusarium sacchari from leaves saponin of Panax ginseng

作　　者：王丹[1] 赵余庆[2] WANG Dan ZHAO Yu-qing(Shenyang Second Hospital of Chinese Medicine, Shenyang 110101, China Shenyang Pharmaceutical University, Shenyang 110016, China)

机构地区：[1]沈阳市第二中医医院,辽宁沈阳110101 [2]沈阳药科大学,辽宁沈阳110016

出　　处：《中草药》2017年第4期648-652,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的利用甘蔗镰孢Fusarium sacchari对人参茎叶皂苷进行生物转化。方法转化产物通过硅胶柱色谱进行分离,经理化常数和光谱分析鉴定化合物的结构。结果从人参茎叶皂苷的Fusarium sacchari转化产物中分离鉴定了10个化合物,分别为20(S)-人参二醇(1)、20(S)-原人参二醇(2)、20(R)-原人参二醇(3)、20(S)-人参三醇(4)、20(S)-原人参三醇(5)、20(R)-原人参三醇(6)、20(S)-原人参二醇-20-O-β-D-吡喃葡萄糖苷(CK)(7)、人参皂苷F1(8)、人参皂苷Rh1(9)和人参皂苷Rg1(10)。结论化合物1~10为首次从人参茎叶皂苷的Fusarium sacchari生物转化产物中分离得到,且Fusarium sacchari可转化人参茎叶皂苷生成稀有抗肿瘤皂苷,是一种具有开发价值的稀有活性菌株。Objective To study the chemical constituents of transformed products by Fusarium sacchari from the leaves saponin of Panax ginseng. Methods Transformation products separated by the process of silica gel column, compounds were identified and elucidated by spectral and chemical methods. Results Ten compounds were isolated from transformed products of Fusarium sacchari from the leaves saponin of P. ginseng and identified as 20 （S）-panaxadiol （1）, 20 （S）-protopanoxa-diol （2）, 20 （R）-protopanoxa-diol （3）, 20 （S）-panaxatriol （4）, 20 （S）-protopanaxatriol （5）, 20 （R）-protopanaxatriol （6）, 20 （S）-protopanoxadiol-20-O-β-D-glucopyranose （7）, ginsenoside-F1 （8）, ginsenoside Rh1（9）, and ginsenoside Rg1 （10）. Conclusion Compounds 1-10 are the compounds of ginsenosides, isolated from transformed products of F. sacchari from the leaves saponin of P. ginseng for the first time. F. sacchari could transform leaves saponin of P. ginseng to rare antitumor saponin, which is a rare active microbial strain with exploitation value.

关 键 词：甘蔗镰孢 生物转化 人参茎叶皂苷 20(S)-人参二醇 20(S)-原人参二醇 人参皂苷F1 

分 类 号：R284.1[医药卫生—中药学]