脂质体平衡透析与液质联用技术筛选脊痛宁片物质基础研究  

Screening of active components of Jitongning Tablet by liposome equilibrium dialysis and LC-MS analysis

作　　者：高迪[1,2,3] 王保军[2,3] 裴玉[2,3] 白雪[2,3] 何毅[2,3] 宋兆辉[2,3] GAO Di WANG Bao-jun PEI Yu BAI Xue HE Yi SONG Zhao-hui(State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China TCM Research Center, Tasly Group Co., Ltd., Tianjin 300410, China State Key Laboratory of Critical Technology in Innovative Chinese Medicine, Tasly Group Co., Ltd., Tianjin 300410, China)

机构地区：[1]中国药科大学天然药物活性组分与药效国家重点实验室,江苏南京210009 [2]天士力控股集团有限公司研究院现代中药开发中心,天津300410 [3]天士力制药集团股份有限公司创新中药关键技术国家重点实验室,天津300410

出　　处：《中草药》2017年第4期668-672,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项课题--面向国际的创新中药大平台建设(2013ZX09402202)

摘　　要：目的研究脊痛宁片有效成分与模拟生物膜的相互作用,预测药物在体内的吸收情况。方法将脂质体作为模拟生物膜,采用脂质体平衡透析与液质联用技术筛选脊痛宁片中与脂质体有相互作用的成分,并考察脂质体质量浓度和缓冲液p H值对脊痛宁片与脂质体互相作用的影响。结果脊痛宁片中共有11个成分与脂质体作用明显,包括4个黄酮类成分,2个萜类成分,4个香豆素类成分,1个苯丙酸类成分。脂质体的质量浓度和缓冲液p H值都对脊痛宁片与脂质体互相作用有一定的影响。结论脂质体平衡透析与液质联用可预测脊痛宁片潜在的活性成分在体内的吸收情况,为其药效物质基础的进一步研究提供依据。Objective To explore the interaction between the effective components of Jitongning Tablet （JT） and liposome biomembrance, in order to predict in vivo absorption of the effective components. Methods Liposome as the model ofbiomembrance, active components were screened by liposome equilibrium dialysis and LC-MS; And the effect of experiment conditions on the interaction of the liposome concentration, and the pH value of buffer were also investigated. Results There were 11 compounds in JT with obvious interaction with liposome, including 4 flavonoids, 2 triterpenoid, 4 coumarins, and 1 phenylpropionic acid. The concentration of liposome and pH value of buffer remarkably affected the interaction between JT and liposome. Conclusion The established method contributes to predicting the multiple components absorption in body, indicating the potentially active compounds and providing reference for further research of pharmacodynamic substance basis.

关 键 词：脊痛宁片 模拟生物膜 脂质体平衡透析 液质联用 活性成分 

分 类 号：R284.2[医药卫生—中药学]