炮制时间对盐补骨脂中10种化学成分的影响  

Determination of 10 components in stir-frying Psoralea Fructus with salt solution by different processing time

作　　者：李凯[1] 许梦莹[1] 周宁[1] 张振凌[1] LI Kai XU Meng-ying ZHOU Ning ZHANG Zhen-ling(Henan University of Chinese Medicine, Zhengzhou 450008, Chin)

机构地区：[1]河南中医药大学,河南郑州450008

出　　处：《中草药》2017年第4期710-713,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目--补骨脂盐炙"入肾"机制研究(81403102)

摘　　要：目的建立同时测定补骨脂Psoralea corylifolia中补骨脂素、异补骨脂素、补骨脂定、补骨脂二氢黄酮甲醚、异补骨脂二氢黄酮、补骨脂二氢黄酮、异补骨脂查耳酮、补骨脂宁、新补骨脂异黄酮和补骨脂酚10种化学成分的方法,并探讨炒制时间对盐补骨脂中10种成分的影响。方法采用UPLC法,色谱条件:C18柱(50 mm×4.6 mm,1.8μm),流动相为乙腈-水,采用梯度洗脱,体积流量1 m L/min,检测波长250 nm,柱温30℃。结果被测定的10种成分色谱峰在15 min分析时间内均有良好的分离度,精密度RSD均小于3%;在室温条件下24 h内稳定;各成分均有较宽的线性范围和良好的线性关系(r≥0.997 0);平均加样回收率96%~105%,RSD均小于3%。结论本方法操作简便,快速,测定结果准确可靠,可用于补骨脂的质量控制。随炒制时间的延长,香豆素类成分呈现先降后升再降趋势,黄酮类、补骨脂酚及10种成分总量有降低趋势。Objective To establish UPLC method for the simultaneous determination of 10 components, such as psoralen, isopsoralen, psoralidin, bavachinin, isobavaenin, corylifolin, isobavachalcone, corylin, neobavaisoflavone, and bakuchiol in Psoralea Fructus, and to study the effect of processing time on 10 components in stir-frying Psoralea Fructus with salt solution. Methods The chromatographic separation was achieved on a C18 column （50 mm×4.6 mm, 1.8 μm） with acetonitrile （A）-water （B） as mobile phase at the flow rate of 1.0 mL/min for gradient elution; The column temperature was 30℃. The determination wavelength was 250 nm. Results The 10 components were well separated within 15 min. The RSD values of reproducibility were less than 3%. The stability was good in 24 h. The linear relationship between the concentration and peak areas of the 10 components was good （r≥0.997 0）. The average recoveries were 96%-105% and the RSD values were all less than 3%. Conclusion The method is simple, reliable and accurate, could be used for the quality control of Psoralea Fructus. With processing time prolonged, the content of coumarins showed first decreased, then increased and last decreased. Flavonoids, bakuchiol and the total content of 10 components above were decreased.

关 键 词：炮制 盐炙 补骨脂 补骨脂素 异补骨脂素 补骨脂定 补骨脂二氢黄酮甲醚 异补骨脂二氢黄酮 补骨脂二氢黄酮 异补骨脂查耳酮 补骨脂宁 新补骨脂异黄酮 补骨脂酚 UPLC 

分 类 号：R286.6[医药卫生—中药学]