长白山地区钝叶瓦松茎提取物的成分及活性研究  

Chemical constituents and activity of stem extract from Orostachys malacophyllus in Changbai Mountain

作　　者：尹秀梅[1] 金春梅[1,2] 王思宏[3,2] YIN Xiu-mei JIN Chun-mei WANG Si-hong(College of Pharmacy, Yanbian University, Yanji 133002, China Analysis and Test Center, Yanbian University, Yanji 133002, China Key Laboratory of Organism Functional Factors of the Changbai Mountains of Ministry of Education, Yanbian University, Yanji 133002, China)

机构地区：[1]延边大学药学院,吉林延吉133002 [2]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 [3]延边大学测试中心,吉林延吉133002

出　　处：《中草药》2017年第5期859-862,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21365023)

摘　　要：目的研究长白山地区钝叶瓦松Orostachys malacophyllus茎中活性成分。方法使用色谱法分离钝叶瓦松甲醇提取物的醋酸乙酯萃取物中的化合物,利用核磁共振波谱、质谱等技术进行结构鉴定,采用CCK-8方法测试活性。结果从钝叶瓦松茎甲醇提取物中分离鉴定出5个成分,分别为苹果酸-1-乙酯(1)、2-(3,4-二羟基苯酰基氧代)-苹果酸单乙酯(2)、2-(3,4-二羟基苯酰基氧代)-苹果酸二甲酯(3)、2-对羟基苯酰基氧代-苹果酸二甲酯(4)、邻苯二甲酸二异丁酯(5)。结论化合物1~5为从该属植物中首次分离得到,其中化合物2是新化合物,命名为瓦松酚酸;其对He La细胞的细胞毒活性的IC_(50)值为111.5μmol/L。Objective To explore the active constituents from the stems of Orostachys malacophyllus in Changbai Mountain. Methods The ethyl acetate fraction of the methanol extract from the stems of O. malacophyllus was separated by chromatographic methods, structure identification was by means of nuclear magnetic resonance and mass spectra, and the activity was determined by CCK-8 experiment. Results Five compounds were isolated and identified as 1-ethyl-2-hydroxysuccinate（1）, 1-ethyl-2-O-[（3,4-dihydroxybenzoyl） oxy]-succinate（2）, 1,4-dimethy-2-O-[（3,4-dihydroxybenzoyl） oxy]-succinate（3）, 1,4-dimethy-2-O-[（4-dihydroxybenzoyl） oxy]-succinate（4）, and diisobutyl phthalate（5）. Conclusion Compounds 1—5 are isolated from the plants of this genus for the first time. Among them, compound 2 is a new compound named orostachysolic acid, and exhibits cytotoxicity against He La cell with IC50 value of 111.5 μmol/L.

关 键 词：钝叶瓦松 苹果酸-1-乙酯 2-(3 4-二羟基苯酰基氧代)-苹果酸单乙酯 邻苯二甲酸二异丁酯 瓦松酚酸 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]