基于UPLC-Triple-TOF/MS方法的三叶青化学成分分析  

Analysis on chemical constituents in Tetrastigma hemsleyanum by UPLC-Triple-TOF/MS

作　　者：曾美玲[1] 沈耐涛 吴赛伟[1] 厉群[1] ZENG Mei-ling SHEN Nai-tao WU Sai-wei LI-Qun(The Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu 322000, Chin)

机构地区：[1]浙江大学医学院附属第四医院,浙江义乌322000

出　　处：《中草药》2017年第5期874-883,共10页Chinese Traditional and Herbal Drugs

摘　　要：目的建立超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-Triple-TOF/MS)快速分析三叶青Tetrastigma hemsleyanum的化学成分。方法采用Agilent Eclipse Plus-C18色谱柱(100 mm×4.6 mm,1.8μm),流动相为乙腈(0.1%甲酸)-水(0.1%甲酸),梯度洗脱,体积流量1 m L/min,柱温30℃。采用电喷雾离子源负离子模式,全扫描模式采集样品数据,根据高分辨数据并结合二级质谱信息快速鉴定三叶青的化学成分。结果推测了三叶青中51种化学成分,主要包括黄酮类、酚酸类、磷脂、糖酯类和苯磺酸类等,并对磷脂、糖酯和苯磺酸类化合物的裂解规律进行总结。结论通过UPLC-Triple-TOF/MS联用技术,为定性分析三叶青的化学成分提供了一种快速、高效的定性分析方法。Objective To establish a quick method of ultra-performance liquid chromatography-quadrupole time-of-fight mass spectrometry（UPLC-Triple-TOF/MS） for the analysis of compounds in Tetrastigma hemsleyanum. Methods The separation was performed on the chromatographic column of Agilent Eclipse Plus-C18（100 mm × 4.6 mm, 1.8 μm）, and the mobile phase was methanol（0.1% formic acid）-0.1% formic acid solution, with a gradient elution at a flow rate of 1 m L/min. The column temperature was 30 ℃, and negative ion mode was used for TOF-MS. Results Fifty-one compounds were identified or tentatively characterized based on the retention time and MS spectra. They are flavonoids, phenolic acids, phospholipids, glycolipids, benzenesulfoic acid, etc. And the preliminary fragmentation rules of phospholipid, glycolipids, and benzenesulfoic acid were summarized. Conclusion The results demonstrate that UPLC-Triple-TOF/MS method is novel, quick, and efficient for the identification of the compounds in T. hemsleyanum.

关 键 词：三叶青 超高效液相色谱串联三重四级杆飞行时间质谱 黄酮 磷脂 酚酸 原花青素 

分 类 号：R284.1[医药卫生—中药学]