球状功能性烟草花叶病毒纳米颗粒的制备及表征  

Preparation and Characterization of Functional Tobacco Mosaic Virus Spherical Nanoparticles

作　　者：吴业红[1,2] 赵霞[1] 胡君[1] 林园[1] 王倩[1,3] WU Yehong ZHAO Xia HU Jun LIN Yuan WANG Qian(Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China University of Chinese Academy of Sciences, Beijing 10049, China University of South Carolina, South Carolina 29208, USA)

机构地区：[1]中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春130022 [2]中国科学院大学,北京100049 [3]美国南卡罗莱纳大学化学与生物化学系,美国南卡罗莱纳州29208

出　　处：《应用化学》2017年第4期379-384,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(21374119,21429401);长春应用化学研究所高分子物理与化学国家重点实验室基金;天津大学精密测试技术及仪器国家重点实验室开放基金资助~~

摘　　要：烟草花叶病毒(TMV)由于其良好的生物相容性、单分散性、多价性、低成本等优点,已作为功能材料的基本构筑单元应用于光电器件、组织工程、疫苗载体,无机纳米材料制备等研究领域。然而,相比于棒状的TMV,球状TMV纳米颗粒无核酸分子,抗环境影响能力更强,比表面积更大。本文利用蛋白质的热致变性原理,对经基因和化学改性后的棒状TMV如半胱氨酸突变体(TMV-Cys)、赖氨酸突变体(TMV-EPMK)和β-环糊精(β-CD)修饰的TMV(TMV-β-CD)进行热变性处理,探究其形成球型纳米颗粒(TMV-SNP)的能力及功能性。结果显示,改性后的TMV经历热变性后可得到形貌均一的球型纳米颗粒,且其暴露在纳米颗粒表面的功能基团Cys、Lys和β-CD仍具有反应活性。Tobacco mosaic virus（TMV） due to its good biocompatibility, monodisperse, multivalent, low cost and other advantages, has been used as the basic building blocks used in optoelectronic devices, tissue engineering, vaccine vector, inorganic material preparation fields. However, compared to the rod like TMV, spherical TMV nanoparticles with no nucleic acid molecules are more resistant to environmental impact and have a larger specific surface area. The genetic and chemical modification of TMV, such as cysteine mutants of TMV （ TMV TMV-Cys）, lysine mutant of TMV （TMV-EPMK） and β-cyclodextrin （β-CD） modified TMV （TMV-β-CD） were conducted by thermal denaturation, and we studied their formation process and functionality. The results shows that the modified TMV can transit into the uniform spherical nanoparticles, and the functional groups exposed to the surface of the nanoparticles still keep their reactive ability.

关 键 词：烟草花叶病毒 热变性 球型纳米颗粒 功能性 突变体 

分 类 号：O636[理学—高分子化学]