提取缓冲液pH值对植物组织中SOD、POD和CAT酶活性的影响  

Effect of pH of Extraction Buffer on SOD,POD and CAT in the Plant Tissue

作　　者：龚屾 石英[1] 韩毅强[1] 高亚梅[1] 郑殿峰[1] 杜吉到[1] Gong Shen Shi Ying Han Yiqiang Gao Yamei Zheng Dianfeng Du Jidao(College of Agronomy, Heilongjiang Bayi Agriculture University, Daqing 163319)

机构地区：[1]黑龙江八一农垦大学农学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2017年第2期8-12,共5页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金项目(31271652);大庆市指导性科技计划项目(zd-2016-041)

摘　　要：植物组织中SOD、POD和CAT活性测定对于研究植物抗逆性极其重要。为了简化实验,利用比色法研究了四种作物在正常水分条件下和干旱胁迫下,不同pH值提取缓冲液对SOD、POD和CAT酶活性的影响。结果表明,SOD不适合在低pH值(6.0)提取缓冲液下提取,POD不适合在pH 7.0的提取缓冲液下提取,CAT可以应用三种提取缓冲液中的任意一种,对酶活性均影响不大。综上所述,SOD、POD和CAT三种酶活均可以应用pH 7.8缓冲液提取,不会显著影响实验结果,提高测定效率。Enzyme activity measurement of SOD,POD and CAT was important for study of plant stress-tolerance. To simplify the experiment, effect of different extraction buffer on enzyme activity measurement of SOD, POD and CAT was analyzed in four crops under normal condition and drought stress by photocolorimetric method. Results showed that the extraction of SOD was unsuitable in tow pH （6.0） buffer,and the extraction of POD was unsuitable in pH 7.0 buffer,and the extraction of CAT. could be carried out in three buffers,which had no effect on enzyme activity of CAT. Above all, pH 7.8 buffer could be applied for extraction of SOD, POD and CAT,and had no obvious effect on result of measurement,and it would improve efficiency of activity measurement of those enzymes.

关 键 词：抗氧化 酶活性 超氧化物歧化酶 过氧化物酶 过氧化氢酶 

分 类 号：Q4-33[生物学—生理学] S-3[农业科学]