盐酸苯胺诱导小鼠皮肤快速衰老模型的建立  

Establishment of Rapid Skin Aging Mouse Model by Aniline Hydrochloride Injection

作　　者：刘军[1] 邱美玉 于楠楠[1] 崔玉东[1] 韩英浩[1] Liu Jun Qiu Meiyu Yu Nannan Cui Yudong Han Yinghao(College of Life Sciences and Technology, Heilongjiang Bayi Agricultural University , Daqing 163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2017年第2期79-85,共7页journal of heilongjiang bayi agricultural university

基　　金：2015研究生创新创业项目(YJSCX2015-Y58)

摘　　要：为研究皮肤衰老机制和抗皮肤衰老药物的筛选奠定基础。采用腹腔注射盐酸苯胺160 mg·kg-1,每3天一次,注射5次的方法建立皮肤衰老小鼠模型。结果显示:注射盐酸苯胺后,Prdx2基因敲除小鼠与野生型小鼠外周血含海因茨小体红细胞数量均上升(P<0.001);小鼠血液活性氧水平升高(P<0.01);皮肤组织中Cyclin D1,Cyclin E,p21,p16的表达量和p38,ERK的磷酸化程度均升高(P<0.01);小鼠真皮层厚度减少。表明酸苯胺可诱导小鼠皮肤提前衰老,且Prdx2可延缓这一作用。In order to set up the experimental foundation for the study of skin aging mechanism and the screening drugs preventing skin aging. Intraperitoneal injections of aniline hydrochloride, 160mg· kg^-1 every three days was used to set up skin premature aging model; Heinz body levels and Heinz body contents were increased after injection （P〈0.001）;the expression of Cyclin D1, Cyclin E, p21 ,p16 and the phosphorylation levels of p38,ERK were higher after injection aniline hydrochloride in the skin （P〈0.01）;the der- mal thickness was decreased after injection. These results showed that Aniline Hydrochloride could induce premature skin aging and Prdx2 could play a protective role.

关 键 词：皮肤衰老 盐酸苯胺 Prdx2 动物模型 小鼠 

分 类 号：Q78[生物学—分子生物学]