豨莶草醇提物对多柔比星致小鼠慢性心肌损伤的保护作用  

Protective Effects of Ethanol Extracts from Siegesbeckiae herba on Doxorubicin-induced Chronic Myocardial Injury in Mice

作　　者：于静[1] 苏素文[2] 杨继章[1] 何林峰[2] 王可欣[2] 张晓飞[2] 

机构地区：[1]河北医科大学第一医院临床药学部,石家庄050031 [2]河北医科大学基础医学院药理教研室,石家庄050017

出　　处：《中国药房》2017年第10期1320-1323,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81273600);河北省自然科学基金资助项目(No.C2011206145);河北省中医药管理局中医药类科研计划课题(No.2012008)

摘　　要：目的:探讨豨莶草(HS)醇提物对多柔比星(DOX)致小鼠慢性心肌损伤的影响。方法:将48只小鼠随机分为3组,即空白对照(Con)组小鼠每天ig 1次蒸馏水(2 mL/100 g),并每周ip 1次生理盐水,持续8周;DOX组小鼠每天ig 1次蒸馏水(2 mL/100g),并每周ip 1次DOX(3 mg/kg),持续8周;DOX+HS醇提物组小鼠每天ig 1次HS(340 mg/kg),并每周ip 1次DOX(3 mg/kg),持续8周。给药结束后,测定小鼠体质量、心脏系数、心功能变化以及血清生化指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肌酸磷酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)及总胆固醇(TC)]水平。结果:与Con组比较,DOX组小鼠体质量减轻(P<0.01);心脏系数升高(P<0.05);心率减慢、R波增高(P<0.05或P<0.01);血清中生化指标均有所升高,其中AST差异有统计学意义(P<0.01)。与DOX组比较,DOX+HS醇提物组小鼠心脏系数降低(P<0.01);心率增加(P<0.01);血清中生化指标均有所降低,其中CK、LDH、TC差异有统计学意义(P<0.01)。结论:HS醇提物对DOX致小鼠慢性心肌损伤有一定的保护作用。OBJECTIVE：To explore the effects of extracts from Siegesbeckiae herba（HS） on doxorubicin（DOX）-induced chronic myocardial injury in mice. METHODS：48 mice were randomly divided into 3 groups. Mice in blank control（Con）group received distilled water once every day,ig,and normal saline（2 mL/100 g）once every day,ip,for 8 weeks;mice in DOX mice received distilled water（2 mL/100 g）once every day,ig,and DOX（3 mg/kg）once every week,ip,for 8 weeks;mice in DOX＋HS group received HS（340 mg/kg）once every day,ig,and DOX（3 mg/kg）once every week,ip,for 8 weeks. After administration,body mass,heart coefficient,cardiac function changes,serum biochemical index levels [alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,creatine phosphokinase（CK）,creatine kinase isoenzyme（CK-MB）,lactate dehydrogenase（LDH）and total cholesterol（TC）] of mice were determined. RESULTS：Compared with Con group,body mass of mice in DOX group was decreased（P〈0.01）;heart coefficient was increased（P〈0.05）;heart rate slowed down,R-wave was increased（P〈0.05 or P〈0.01）;serum biochemical indexed were increased,there was significant difference in AST（P〈0.01）. Compared with DOX group,heart coefficient of mice in DOX ＋ HS group was decreased（P〈0.01）;heart rate was increased（P〈0.01）;serum biochemical indexes were decreased,there was significant differences in CK,LDH,TC（P〈0.01）. CONCLUSIONS：HS has certain protective effects on DOX-induced chronic myocardial injury in mice.

关 键 词：豨莶草醇提物 多柔比星 慢性心肌损伤 小鼠 

分 类 号：R969.4[医药卫生—药理学]