藏药甘肃马先蒿有效成分提取工艺研究  

Study on the Extraction Technology for Active Constituents of Tibetan Medicine Pedicularis kansuensis

作　　者：曹馨元[1,2] 李茂星[1,2] 毛婷[1,2] 陶锐 王先敏[1] 刘延彤[1] 马强[4] 

机构地区：[1]兰州军区兰州总医院药剂科/全军高原环境损伤防治重点实验室,兰州730050 [2]兰州大学药学院,兰州730000 [3]银川市妇幼保健院药剂科,银川750001 [4]兰州军区兰州总医院消化科,兰州730050

出　　处：《中国药房》2017年第10期1357-1360,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81374019);甘肃省中医药管理局科研课题(No.GZK-2015-59);全军医药卫生科研基金课题(No.CLZ15JA05)

摘　　要：目的:优化藏药甘肃马先蒿提取工艺并比较不同产地甘肃马先蒿中2种成分毛蕊花糖苷及异毛蕊花糖苷的含量差异。方法:以毛蕊花糖苷、异毛蕊花糖苷及干膏得率为综合评价指标,通过单因素试验及正交试验对提取溶剂、溶剂量、提取时间及提取次数进行考察以优化提取工艺,并进行验证试验。比较甘肃、青海及四川3个产地的甘肃马先蒿中2种成分毛蕊花糖苷及异毛蕊花糖苷的含量。结果:最优提取工艺为加8倍量的50%乙醇提取3次,每次90 min。验证试验中毛蕊花糖苷、异毛蕊花糖苷含量及干膏得率平均值分别为3.49%、1.26%、37.99%(RSD分别为1.28%、1.32%、1.97%,n=3)。以青海产甘肃马先蒿中毛蕊花糖苷及异毛蕊花糖苷含量相对较高。结论:优化的提取工艺合理、稳定、可行,不同产地的甘肃马先蒿中指标成分的含量存在一定的差异。本试验可为甘肃马先蒿提取物的开发利用、药材品质评价的深入研究提供依据。OBJECTIVE：To optimize the extraction technology of Tibetan medicine Pedicularis kansuensis and compare content of verbascoside and isoverbascoside differences in P. kansuensis from various habitats. METHODS：Using verbascoside and isoverbascoside and dry paste yield as comprehensive evaluation indexes,single factor test and orthogonal test were used to investigate the extraction solvent,solvent dosage,extraction time and times to optimize extraction technology,and the verification test was conducted. Contents of the 2 constituents verbascoside and isoverbascoside in P. kansuensis from Gansu,Qinghai and Sichuan werecompared. RESULTS：The optimal extraction technology was as follows as 8-fold 50% ethanol,extraction for 3 times,90 min each time. The verification results showed that the average contents of verbascoside and isoverbascoside were 3.49%（RSD=1.28%,n=3）,1.26%（RSD=1.32%,n=3）,and average dry paste yields were 37.99%（RSD=1.97%,n=3）. The contents of verbascoside and isoverbascoside in P. kansuensis from Qinghai were relatively higher. CONCLUSIONS：Optimized extraction technology is reasonable,stable,feasible;the contents of index constituents in P. kansuensis from different habitats have certain differences. The study can provide scientific evidence for the development and utilization of extraction,and the in-depth study of quality evaluation for medicinal material.

关 键 词：甘肃马先蒿 毛蕊花糖苷 异毛蕊花糖苷 正交试验 提取工艺 含量比较 

分 类 号：R284.2[医药卫生—中药学]