大量元素对多花黄精不定芽生长和次生代谢物含量的影响  

Effects of Macroelement Concentration on Growth and Secondary Metabolites Contents of Polygonatum cyrtonema's Adventitious Bud

作　　者：周新华[1] 肖智勇 黄拯[1] 曾满生[1] 李峰卿[1] 武晓玉[1] 

机构地区：[1]中国林业科学研究院亚热带林业实验中心,江西分宜336600 [2]宜春市林业科学研究所,江西宜春336000

出　　处：《西南林业大学学报（自然科学）》2017年第2期74-79,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017MB024)资助

摘　　要：以MS+NAA 1.0 mg/L+KT 1.5 mg/L为基本培养基,多花黄精根茎芽为材料,研究MS培养基中5种大量元素浓度对多花黄精根茎芽生长及黄精多糖和总皂苷2种次生代谢物合成的影响。结果表明:最适合多花黄精根茎芽生长的培养基中硝酸铵和硝酸钾浓度为MS培养基标准浓度的1.5倍,磷酸二氢钾、硫酸镁和氯化钙浓度为MS培养基标准浓度的2倍;最适合多花黄精总多糖合成的培养基中磷酸二氢钾浓度为MS培养基标准浓度的2倍,其他元素均是MS培养基中的标准浓度;最适合多花黄精总皂苷合成的培养基中5种大量元素浓度均为MS培养基标准浓度。Taken germinative rootstock of Polygonatum cyrtonema as the material. And the experiment was based on MS ＋ NAA 1.0mg/L＋ KT 1.5mg/L medium. It was investigated the complex effect that made by the concentration of the 5 kinds of macroelements in MS medium on the growth of germinative rootstock of P.cyrtonema and the 2 kinds of secondary metabolites include amylose and total saponins. The results indicated that in the most suitable medium for the growth of P.cyrtonema germinative rootstock, the concentration of NH4NO3 and KNO3 was 1.5 times as strong as the standard concentration of MS medium. The concentration of KH2PO4, MgSO4 and CaCl2 was 2 times as strong as the standard concentration of MS medium. In the most suitable medium that synthesized by total anylose, the concentration of KH2PO4 was 2 times as strong as the standard concentration of MS medium. In addition, the other elements were all as strong as the standard concentration of MS medium. In the most suitable medium that synthesized by total saponins, the concentration of 5 kinds of macroelements were MS medium′s standard concentration.

关 键 词：多花黄精 组织培养 大量元素 次生代谢物 根茎芽 

分 类 号：S722.37[农业科学—林木遗传育种]