白花木瓜多聚原花青素降解条件优化  

Optimization for Degradative Process of Polymeric Proanthocyanidins from Chaenomeles cathayensis

作　　者：张霞[1,2] 姜力[1,2] 李娜[1,2] 罗旭璐 张加研[1] 赵平[1,2] 

机构地区：[1]西南林业大学云南省木材胶黏剂及胶合制品重点实验室,云南昆明650224 [2]西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明650224

出　　处：《西南林业大学学报（自然科学）》2017年第2期209-215,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31260163)资助;云南省教育厅科学研究基金重大专项(ZD2010006)资助

摘　　要：以白花木瓜多聚原花青素的降解主产物(-)-表儿茶素-(4β-8)-(-)-表没食子儿茶素3-O-没食子酸酯(1)的含量为指标,采用单因素试验考察反应温度、反应时间、物料比(多聚原花青素/茶多酚,PPC/TP)和盐酸浓度对化合物1生成量的影响,并通过响应面法对白花木瓜PPC降解条件进行优化。结果表明:白花木瓜PPC的最佳降解条件为:反应温度70℃,反应时间81.5 min,PPC/TP比为1.5∶1.0,盐酸浓度为1%。在此条件下,化合物1的含量为601.093mg/mL,与理论预测值(606.103 mg/mL)较为接近,表明采用响应面法优化白花木瓜PPC降解条件的方法可行。Using the content of （-）-epicatechin-（4β-8）-（-）-epigallocatechin 3-O-gallate （1）, the main degradative product of polymeric proanthocyanidins （PPC） from Chaenomeles cathayensis with tea polyphenols （TP） as an indicator, the effects of reaction temperature, reaction time, PPC/TP ratio and HCl concentration on the content of 1 were investigated by single factor experiment, and the degradative process was opyimized by response surface methodology. The results showed that the optimum degradation condition of PPC were established as 70℃ of reaction temperature, 81.5min of reaction time, 1.5∶1 of PPC/TP ratio and 1% of HCl concentration. Under this optimal condition, the actual content of 1 was 601.093g/mL that was close to the theoretic value （606.103g/mL）, indicated that the response surface method to optimize the degradative process of PPC from C.cathayensis was feasible.

关 键 词：白花木瓜 多聚原花青素 降解 优化 

分 类 号：S785[农业科学—林学]