去铁斯若治疗去卵巢小鼠铁蓄积骨量下降  

Effects of deferasirox on bone mass and construction in ovariectomy mice with iron accumulation

作　　者：陈斌[1] 汪升[1] 王啸[1] 叶俊星[2] 刘禄林[1] 高焱[1] 徐又佳[1] 

机构地区：[1]苏州大学附属第二医院苏州大学骨质疏松诊疗技术研究所,苏州215000 [2]无锡市第三人民医院骨科,无锡214000

出　　处：《中华骨质疏松和骨矿盐疾病杂志》2017年第2期140-145,共6页Chinese Journal Of Osteoporosis And Bone Mineral Research

基　　金：国家自然科学基金(81572179);江苏省临床医学科技专项(BL2014044);苏州市民生科技项目(SS201634);苏州市骨质疏松症临床医学中心项目(SZZXJ201504);苏州大学附属第二医院优势临床学科群项目(XKQ2015001)

摘　　要：目的观察去铁斯若(deferasirox,DFR)对加枸橼酸铁铵(ferric ammonium citrate,FAC)铁蓄积绝经后小鼠骨丢失的治疗作用。方法 32只2月龄小鼠采用数字表法随机分为4组。假手术(Sham)组采用0.9%氯化钠注射液干预;去卵巢(ovariectomy,OVX)对照组切除双侧卵巢;去卵巢(OVX+FAC)组切除双侧卵巢后给予枸橼酸铁铵80 mg/kg(溶于0.9%氯化钠注射液)腹腔注射,每周3次,共持续5周;去卵巢加枸橼酸铁铵加去铁斯若(OVX+FAC+DFR)组在枸橼酸铁铵干预基础上给予去铁斯若100 mg/kg(溶于0.9%氯化钠注射液)灌胃,每周5次,持续6周。最后检测各组铁蛋白水平、骨铁含量以及股骨远端松质骨micro-CT扫面分析和三维重建。结果血清铁蛋白、骨Perl's染色提示OVX+FAC组铁蛋白(220.7±28.3)μg/L,显著高于Sham组(112.8±6.0)μg/L和OVX组(103.8±22.4)μg/L,DFR干预后OVX+FAC+DFR组铁蛋白(151.5±36.1)μg/L有所下降,差异有统计学意义(P<0.05)。三维重建示OVX+FAC组小鼠骨小梁稀疏,骨密度(0.074±0.010)mg/mm^3减低,DFR干预后骨小梁稍有增加,骨密度(0.110±0.015)mg/mm^3显著恢复。结论 DFR可部分逆转铁蓄积导致的骨量下降,从而提高骨密度,改善骨微结构。Objective To explore the effect of deferasirox （DFR） on bone mass and construction in ovariectomy （OVX） mice with iron accumulation by feeric ammonium citrate （FAC）. Methods Thirty-two month old C57 mice were randomly divided into 4 groups. Sham group received saline treatment. Mice in OVX group were experienced ovariectomy. Mice in OVX ＋ FAC group received intraperitoneal FAC 80 mg/kg for 5 weeks after ovarieetomy. As tot mice in OVX ＋ FAC ＋ DFR group, after ovariectomy and FAC treatment, the iron accumulated mice received DFR treatment for 6 weeks. At last, we detected serum ferritin levels, bone iron levels and bone three-dimensional reconstruction. Results Serum ferritin levels and Perl＇s blue staining of bone suggested that iron levels in mice of OVX ＋ FAC group （ferritin 220.7 ± 28.3μg/L） were significantly higher than those in Sham group （ 112.8 ± 6.0 μg/L） and OVX group （ 103.8 ± 22.4 μg/L）. After the treatment of DFR, the iron levels were decreased. 3D reconstruction suggested decreased cancellous bone parameters and bone mineral density （0. 074 ± 0. 010 mg/mm3 ）. After DFR treatment, the bone mineral density （0. 110 ± 0. 016 mg/mm3） and trabecular bone were increased. Conclusion DFR could improve bone mineral density and improve bone micro-construction by reducing iron accumulation.

关 键 词：去铁斯若 铁蓄积 骨质疏松 

分 类 号：R681[医药卫生—骨科学]