七氟醚后处理对大鼠心肌缺血再灌注时Pim-1激酶表达的影响：离体实验  

Effect of sevoflurane postconditioning on expression of Pim-1 kinase during myocardial ischemia-reperfusion in rats： an in vitro experiment

作　　者：陈慧娟[1] 王大亮[1] 王慧 田环环 刘金东 

机构地区：[1]徐州医科大学江苏省麻醉学重点实验室、江苏省麻醉与镇痛应用技术重点实验室,221004 [2]徐州医科大学附属医院麻醉科,221006

出　　处：《中华麻醉学杂志》2016年第12期1457-1461,共5页Chinese Journal of Anesthesiology

摘　　要：目的 评价七氟醚后处理对大鼠心肌缺血再灌注时Pim-1激酶表达的影响.方法 雄性SD大鼠,体重250～ 300 g,建立Langendorff离体心脏灌注模型36个,采用随机数字表法,将离体灌注心脏分为3组（n=12）：对照组（C组）、缺血再灌注组（I/R组）和七氟醚后处理组（SP组）.采用缺血30 min再灌注120 min的方法制备心肌缺血再灌注损伤模型.再灌注即刻SP组用含3％七氟醚的K-H液灌注15 min.分别于平衡灌注末（T0）、再灌注30 min（T1）、60 min（T2）、90 min（T3）和120 min（T4）时记录心率（HR）、左室舒张末压（LVEDP）、左室发展压（LVDP）、左心室内压上升最大速率（＋dp/dtmax）和左心室内压下降最大速率（-dp/dtmax）.T2时取心肌组织,采用Western blot法检测胞浆和线粒体Pim-1激酶、Bcl-2、细胞色素c（Cyt c）的表达水平;T4时采用TTC染色法确定心肌梗死体积;电镜下观察线粒体超微结构.结果 与C组比较,I/R组T1～4时HR、LVDP、＋dp/dtmax和-dp/dtmax降低,LVEDP升高,心肌梗死体积增大,胞浆及线粒体Pim-1激酶和Bcl-2表达下调,胞浆Cyt c表达上调,线粒体Cyt c表达下调（P＜0.05）;与I/R组比较,SP组T1～4时HR、LVDP、＋dp/dtmax和-dp/dtmax升高,LVEDP降低,心肌梗死体积减小,胞浆及线粒体Pim-1激酶和Bcl-2表达上调,胞浆Cyt c表达下调,线粒体Cyt c表达上调（P＜0.05）;SP组线粒体损伤程度轻于I/R组,而重于C组.结论 七氟醚后处理减轻大鼠心肌缺血再灌注损伤的机制可能与上调Pim-1激酶表达有关.Objective To evaluate the effect of sevoflurane postconditioning on the expression of Pim-1 kinase during myocardial ischemia-reperfusion （I/R） in rats.Methods Male Sprague-Dawley rats,weighing 250-300 g,were used in this study.After the animals were anesthetized,their hearts were immediately removed and retrogradely perfused with an oxygenated K-H solution at 37 ℃ in a Langendorff apparatus.Thirty-six isolated rat hearts were assigned into 3 groups （n=12 each） using a random number table：control group （group C）,group I/R and sevoflurane postconditioning group （group SP）.The hearts were subjected to ischemia for 30 min followed by 120 min of reperfusion to establish the model of myocardial I/R injury.In group SP,the hearts were perfused with K-H solution saturated with 3% sevoflurane for 15 min starting from the beginning of reperfusion.Heart rate （HR）,left ventricular end-diastolic pressure （LVEDP）,left ventricular developed pressure （LVDP） and the maximum rate of increase and decrease of left ventricular pressure （±dp/dtmax）were recorded at the end of equilibration and 30,60,90and 120 min of reperfusion.Myocardial tissues were obtained at T2 for determination of the expression of Pim-1 kinase,Bcl-2 and cytochrome c （Cyt c） in cytoplasm and mitochondria by Western blot.The hearts were selected at T4 for determination of myocardial infarct size （by TTC staining） and for examination of mitochondrial ultrastructure （with transmission electron microscope）.Results Compared with group C,HR,LVDP and ±dp/dtmax were significantly decreased,and LVEDP was increased at T1-4,the myocardial infarct size was enlarged,the expression of Pim-1 and Bcl-2 in cytoplasm and mitochondria was down-regulated,the expression of Cyt c in cytoplasm was up-regulated,and the expression of Cyt c in mitochondria was down-regulated in group I/R （P〈0.05）.Compared with group I/R,HR,LVDP and ＆#177;dp/ dtmax were significantly increased,and LVEDP was decreased at T1-4,the myocardial infarct s

关 键 词：原癌基因蛋白质类 麻醉药 吸入 缺血后处理 心肌再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]