瑞芬太尼诱发切口痛小鼠痛觉过敏时脊髓水通道蛋白4表达的变化  

Changes in expression of spinal aquaporin-4 during remifentanil-induced hyperalgesia in a mouse model of incisional pain

作　　者：吴碧玲[1] 赖忠盟[1] 陈文华[1] 张良成[1] 林鹏焘[1] 

机构地区：[1]福建医科大学附属协和医院麻醉科,福州市350001

出　　处：《中华麻醉学杂志》2016年第12期1462-1464,共3页Chinese Journal of Anesthesiology

基　　金：福建省自然科学基金面上项目（2013J01307）

摘　　要：目的 评价瑞芬太尼诱发切口痛小鼠痛觉过敏时脊髓水通道蛋白4（AQP4）表达的变化.方法 清洁级健康成年雄性CD1小鼠72只,体重25～30 g,采用随机数字表法分为4组（n=18）：对照组（C组）皮下泵注生理盐水;切口痛组（I组）制备小鼠切口痛模型;瑞芬太尼组（R组）皮下泵注瑞芬太尼80 μg/kg 30 min,泵注速率0.8 ml/h;瑞芬太尼＋切口痛组（R＋I组）皮下泵注瑞芬太尼后制备切口痛模型.于模型制备前1 d（T0）、模型制备后6h、1、2和7d（T1～4）时测定热缩足潜伏期（TWL）和机械缩足反应阈（MWT）.于T3时测定痛阈后各组随机处死12只小鼠取脊髓腰段,采用Western blot法检测AQP4的分布和表达.结果 与C组比较,R组和R＋I组T1～3时TWL缩短,T2～4时MWT降低,I组、R组和R＋I组T3时脊髓AQP4表达上调（P＜0.05）;与I组比较,R组T23时TWL缩短,T2～4时MWT降低,R＋I组T1～3时TWL缩短,T2～4时MWT降低,T3时脊髓AQP4表达上调（P＜0.05）.结论 瑞芬太尼诱发切口痛小鼠痛觉过敏的机制与脊髓AQP4表达上调有关.Objective To evaluate the changes in the expression of spinal aquaporin-4 （AQP4） during remifentanil-induced hyperalgesia in a mouse model of incisional pain.Methods Seventy-two pathogen-free healthy adult male CD1 mice,weighing 25-30 g,were divided into 4 groups （n=18 each） using a random number table：control group （group C）,incisional pain （group I）,remifentanil group （group R） and remifentanil plus incisional pain group （group R＋I）.Normal saline was infused subcutaneously in group C.An incision was made in the left hind paw in group I.Remifentanil 80 μg/kg was subcutaneously infused for 30 min at a rate of 0.8 ml/h in group R.Remifentanil was infused subcutaneously before establishment of the model in group R＋I.The thermal paw withdrawal latency （TWL） and mechanical paw withdrawal threshold （MWT） were measured at 1 day before establishment of the model （T0） and 6 h and 1,2 and 7 days after establishment of the model （T1-4）.After measurement of the pain threshold at T3,12 animals were sacrificed randomly,and the lumbar segment of the spinal cord was removed for determination of the distribution and expression of AQP4 by Western blot.Results Compared with group C,the TWL was significantly shortened at T1-3,and the MWT was decreased at T2-4 in R and R ＋ I groups,and the expression of AQP4 was significantly up-regulated at T3 in I,R and R＋I groups （P〈0.05）.Compared with group I,the TWL was significantly shortened at T2,3,and the MWT was decreased at T2.4 in group R,and the TWL was significantly shortened at T1-3,the MWT was decreased at T2.4,and the expression of AQP4 was up-regulated at T3 in group R＋I （P〈0.05）.Conclusion The mechanism by which remifentanil induces hyperalgesia is related to up-regulation of AQP4 expression in the spinal cord in a mouse model of incisional pain.

关 键 词：哌啶类 疼痛 手术后 痛觉过敏 脊髓 水通道蛋白质4 AQUAPORIN 4 

分 类 号：R614[医药卫生—麻醉学]