姜黄素对大鼠内毒素性肺损伤时TAK1磷酸化的影响  

Effect of curcumin on phosphorylation of TAK1 during endotoxin-induced lung injury in rats

作　　者：魏磊[1] 杨芬[2] 赵建华[2] 杨建平[1] 

机构地区：[1]苏州大学附属第一医院麻醉科,215006 [2]南京医科大学附属苏州医院麻醉科,215002

出　　处：《中华麻醉学杂志》2016年第12期1497-1499,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价姜黄素对大鼠内毒素性肺损伤时转化生长因子-β激活激酶1（TAK1）磷酸化的影响.方法 清洁级健康雄性SD大鼠18只,体重180～ 220 g,采用随机数字表法分为3组（n=6）：对照组（C组）、肺损伤组（LI组）和姜黄素组（CUR组）.采用气管内滴注脂多糖5 mg/kg的方法制备大鼠内毒素性肺损伤模型.CUR组于制备模型前30 min时腹腔注射姜黄素200 mg/kg,LI组注射等容量二甲基亚砜.于滴注脂多糖后12 h时处死大鼠取肺组织,采用Western blot法检测TAK1和磷酸化TAK1（p-TAK1）的表达,取右上叶肺组织,称湿干重,计算肺湿干重（W/D）比值.结果 与C组比较,LI组肺W/D比值升高,TAK1和p-TAK1表达上调（P＜0.05）;与LI组比较,CUR组肺W/D比值降低,TAK1和p-TAK1表达下调（P＜0.05）.结论 姜黄素减轻大鼠内毒素性肺损伤的机制与抑制肺组织TAK1磷酸化有关.Objective To evaluate the effect of curcumin on the phosphorylation of transforming growth factor-β-activated kinase 1 （TAK1） during endotoxin-induced lung injury in rats.Methods Eighteen pathogen-free healthy male Sprague-Dawley rats,weighing 180-220 g,were divided into 3 groups （n =6 each） using a random number table：control group （group C）,lung injury group （group LI） and curcumin group （group CUR）.Lung injury was induced by intra-tracheal instillation of lipopolysaccharide 5 mg/kg in 1 ml of normal saline in LI and CUR groups.Curcumin 200 mg/kg was injected intraperitoneally at 30 min before establishment of the model in group CUR,while the equal volume of dimethyl sulfoxide was given instead in group LI.The rats were sacrificed at 12 h after administration of lipopolysaccharide or normal saline,and lungs were removed for determination of the expression of TAK1 and phosphorylated TAK1 （p-TAK1） in lung tissues.The right upper lobe of the lung was removed for determination of wet/dry weight ratio （W/D ratio）.Results Compared with group C,the W/D ratio was significantly increased,and the expression of TAK1 and p-TAK1 was up-regulated in group LI （P〈0.05）.Compared with group LI,the W/D ratio was significantly decreased,and the expression of TAK1 and p-TAK1 was down-regulated in group CUR （P〈0.05）.Conclusion The mechanism by which curcumin attenuates endotoxin-induced lung injury is related to inhibition of the phosphorylation of TAK1 in lung tissues of rats.

关 键 词：姜黄素 内毒素血症 呼吸窘迫综合征 成人 转化生长因子Β 

分 类 号：R285.5[医药卫生—中药学]