FKBP51在结直肠癌与正常组织中的表达差异及与临床病理特征的关系  

Expression difference of FKBP51 in colorectal cancer and normal tissues and its relationship with clirdcopathological features

作　　者：夏志秀[1] 王昌亮[2] 韩彦槊[1] 陈春生[1] 张国华[2] 冯勇[1] 

机构地区：[1]中国医科大学附属盛京医院普通外科,沈阳110004 [2]中国医科大学法医学院,沈阳110122

出　　处：《中国医师杂志》2017年第3期362-366,共5页Journal of Chinese Physician

基　　金：辽宁省科技厅科学技术计划项目（2011225019）

摘　　要：目的探讨FK506结合蛋白51（FKBP51）在结直肠腺癌和正常结直肠组织中的表达水平差异，以及结直肠腺癌中FKBP51表达水平与临床病理特征之间的相关性，明确FKBP51是否参与结直肠癌的发生发展。方法采用免疫组化染色（SP法）和Western蛋白印迹方法检测31例结直肠腺癌患者肿瘤组织和距离肿瘤5 cm处正常结直肠组织中FKBP51表达水平差异，并结合患者临床病理资料，用统计学四格表资料的χ2检验进行结果分析。结果肿瘤组织中FKBP51高表达率为74.19%（23/31）；正常组织中FKBP51高表达率为9.68%（3/31），差异有统计学意义。肿瘤组织中FKBP51表达水平与结直肠癌患者的性别（P＝0.771）、年龄（P＝0.474）、肿瘤部位（P＝0.213）、分化程度（P＝0.318）、淋巴结转移（P＝0.124）、远处转移（P＝0.318）、临床分期（P＝0.171）无明显相关性；与肿瘤大小（P＝0.049）、浸润深度（P＝0.031）有关，差异有统计学意义。结论FKBP51在结直肠癌中表达较强，在正常结直肠组织中表达较弱，并且随着肿瘤增大和浸润程度加深，FKBP51表达增强，说明FKBP51参与结直肠癌的发生发展，可能成为结直肠癌个体化治疗的新靶点。Objective To explore the difference of the expression level of FK506 Binding Protein 51 （FKBP51 ） in colorectal adenocareinoma and normal colorectal tissues, and the correlation between FK- BP51 expression level and clinicopathological characteristics, and to clarify whether FKBP51 is involved in the occurrence and development of colorectal cancer. Methods By immunohistochemical staining [ streptavidin-peroxidase （SP） method] and Western blotting methods tested 31 cases of colorectal cancer tumor tissues and normal colorectal tissues far from tumor 5 cm, and explored the expression level difference of FKBP51. Combined with clinical data of patients, results were analyzed by statistical method X2 test of four case table data. Results The high expression rate of FKBP51 in tumor tissues was 74. 19% （23/31 cases）, while the high expression rate of FKBP51 in normal tissue was 9.68% （3/31 cases）. The difference was significant. The expression level of FKBP51 in patients with colorectal carcinoma had no obvious correlation with gender （ P = 0. 771 ）, age （ P = 0. 474 ）, tumor location （ P = 0. 213 ）, degree of differentiation （P =0. 318）, lymph node metastasis （P =0. 124） ,distant metastasis （P =0. 318） and clinical stage （P = 0. 171 ） ; and the tumor size （P =0. 049） , depth of invasion related （P =0. 031 ）, the difference was statistically significant. Conclusions The expression of FKBP51 in colorectal cancer was strong, while weak expression in normal eolorectal tissues. With the increase of tumor infiltration and deepening, the expression of FKBP51 became stronger, which indicated that FKBP51 participated in the genesis and development of colorectal cancer, and it might become a new target for individual therapy of colorectal cancer.

关 键 词：他克莫司结合蛋白质类/代谢 结直肠肿瘤/代谢 

分 类 号：R735.34[医药卫生—肿瘤]