双叶清肺止咳糖浆的质量标准研究  

Determination of Total Flavonoids Content in Shuangye Qingfei Cough Syrup by Colorimetric Method

作　　者：崔瑾瑾 

机构地区：[1]襄阳市中医医院药学部,湖北襄阳441000

出　　处：《湖北医药学院学报》2016年第5期476-478,共3页Journal of Hubei University of Medicine

摘　　要：目的：建立双叶清肺止咳糖浆的质量标准。方法：采用薄层色谱法对方中的炙麻黄、浙贝母进行定性鉴别;采用比色法,以Na NO2-Al Cl3-Na OH显色系统为显色剂,芦丁为对照品,对方中的总黄酮进行定量鉴别。结果：双叶清肺止咳糖浆中的炙麻黄、浙贝母薄层色谱鉴别斑点清晰,芦丁对照品在8.32-66.56μg·m L-1范围内线性良好,平均回收率为98.8%,RSD为1.25%（n=5）。结论：本方法简易可行,准确性、重现性好,可用于双叶清肺止咳糖浆的质量控制。Objective To establish the quality criterion for Shuangye qingfei cough syrup. Methods Thin layer chromatography（ TLC） method was used to identify Chinese Ephedra,Thunberg Fritillary Bulb. Total flavonoids were determined by Na NO2-Al Cl3-Na OH colorimetry using rutin as control. Results TLC spots were clear and well separated without negative interference,a good linear relationship was achieved at the ranges of 8.32 ~ 66.56 μg·m L-1for rutin with an average recovery of 98.8%,RSD = 1.25%（ n = 5）. Conclusion The method is simple,accurate and reproducible. It can be used for the quality control of Shuangye qingfei cough syrup.

关 键 词：双叶清肺止咳糖浆 质量标准 总黄酮 比色法 

分 类 号：R286.0[医药卫生—中药学]