内皮祖细胞移植对急性肺损伤大鼠的抗炎效应  

Anti-inflammatory effect of endothelial progenitor cells transplantation in rats with acute lung injury

作　　者：李辉[1] 苏伟[1] 许东琳[1] 傅永鸿[1] 陈伟明[1] 周慧[1] 杨自力[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院重症医学科,广东广州510180

出　　处：《广州医科大学学报》2016年第5期15-17,共3页Academic Journal of Guangzhou Medical University

基　　金：广州市科技攻关项目(201300000196)

摘　　要：目的:研究内皮祖细胞(EPC)移植对急性肺损伤(ALI)大鼠的抗炎效应。方法:将60只雄性SD大鼠随机分为对照组、ALI组、EPC组,每组各20只。ALI组和EPC组采用LPS(5 mg/kg)静脉注射制备ALI模型,EPC组在造模成功半小时后予EPC悬液移植,观察3组干预过程中血清TNF-α、IL-1β、IL-6和IL-10水平的变化,以及肺湿/干重比值(W/D)、TLR9表达和NF-κB活性的变化。结果:造模后ALI组和EPC组大鼠血清TNF-α、IL-1β、IL-6、和IL-10水平均显著升高(P<0.05)。造模7 d后,EPC组大鼠TLR9表达和NF-κB活性显著低于ALI组(P<0.05),血清TNF-α、IL-1β、IL-6水平也低于ALI组(P<0.05)。结论:EPC移植可以减轻ALI大鼠的炎症反应,具有一定的抗炎效应。Objective： To investigate the anti-inflammatory effect of endothelial progenitor cells （EPCs） transplantation in rats with acute lung injury. Methods： Sixty male SD rats were randomly divided into the control group, ALl group and EPC group （n：20 each）.The ALl group and EPC group were treated with LPS （5 mg/kg） intravenously to prepare ALl model. The EPC group was given cell suspension transplantation at half an hour after successful modeling. The changes of serum TNF-α, IL-113, IL-6 and IL-10 levels, wet-to-dry lung weight （W/D） ratio, TLR9 expression and NF-KB activity during the intervention were determined in the three groups. Results： The serum TNF-α, IL-113, IL-6, and IL-10 levels in the ALl group and EPC group were significantly increased （P〈0.05）. At 7 d after modeling, the expression of TLR9 and the activity of NF-KB in the EPC group were significantly lower than those in the ALI group （P〈0.05） , and the serumTNF-a, IL-113 and IL-6 levels inthe EPC group were also lower than those in the ALl group （ P〈0.05 ）. Conclusion ： EPCs transplantation can relieve inflammatory reaction in rats with acute lung injury with anti-inflammatory effect.

关 键 词：内皮祖细胞移植 急性肺损伤 大鼠 细胞因子 

分 类 号：R563[医药卫生—呼吸系统] Q813.3[医药卫生—内科学]