一氧化碳释放分子-2对小鼠广泛耐药鲍曼不动杆菌肺炎的疗效观察  

Effect of carbon monoxide-releasing molecule (CORM-2) in mouse model of pneumonia caused by extremely-drug resistant Acinetobacter baumannii

作　　者：秦帅[1] 王晓丽[1] 潘婷婷[1] 谭若铭[1] 李梅玲[1] 李磊[1] 瞿洪平[1] 黄洁[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院重症医学科,上海200025

出　　处：《中国感染与化疗杂志》2016年第5期583-588,共6页Chinese Journal of Infection and Chemotherapy

基　　金：上海市科委医学引导项目(134119b0200)

摘　　要：目的评价一氧化碳释放分子-2(CORM-2)对小鼠广泛耐药鲍曼不动杆菌(XDRAB)肺炎的疗效。方法通过体外抑菌试验检测CORM-2对XDRAB生长的抑制作用。再通过建立XDRAB肺炎小鼠模型,观察CORM-2对肺炎小鼠的抗菌、抗炎效果。结果 CORM-2显著下调XDRAB肺炎小鼠肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、高迁移率族蛋白B1(HMGB1)和抗炎因子IL-10的水平,抑制肺组织髓过氧化物酶(MPO)与一氧化氮(NO)的表达,减轻肺组织病理损伤,同时显著降低了肺组织内细菌负荷量。结论 CORM-2在体外和体内对XDRAB均具有显著的抑菌作用,同时在体内发挥重要的抗炎、抗氧化作用。Objective To evaluate the efficacy of carbon monoxide-releasing molecule （CORM-2） in mouse model of pneumonia caused by extremely-drug resistant Acinetobacter baumannii （XDRAB）. Methods To investigate the effect of CORM-2 on XDRAB proliferation in the bacteriostatic test in vitro. Then the mouse model of pneumonia was established by XDRAB challenge. The mice in experimental group were treated with CORM-2 by nasal insufflation. The anti-inflammatory and bacteriostatic effects of CORM-2 were analyzed by comparing with the non-treatment group. Results Compared to the control group, CORM-2 treatment significantly down-regulated the inflammatory factors of TNF-α,/L-6, HMGBI and IL-10 in serum, inhibited the expressions of myeloperoxidase and nitrogen monoxide in lung tissues, alleviated pathological lung damage, and reduced bacterial load in lung tissues. Conclusions CORM-2 has significant bacteriostatic effect on XDRAB in vitro and in vivo. It also plays an important role in the anti-inflammatory and antioxidant process in vivo.

关 键 词：一氧化碳释放分子 鲍曼不动杆菌 肺炎 小鼠 

分 类 号：R446.5[医药卫生—诊断学]