养肝益水颗粒对自发性高血压大鼠肾小球足细胞TGF-β_1、p27蛋白及其基因表达的影响  

Effect of Yanggan Yishui Granule on Protein and Gene Expression of Transforming Growth Factor-β_1 and p27 in Glomerular Podocytes in Spontaneously Hypertensive Rats

作　　者：杨帆[1] 戴小华[1] 朱涛[1] 付军[1] 王银燕[1] 戚先伟 梁国庆[1] 董梅[1] 邵正斌[1] YANG Fan DAI Xiao-hua ZHU Tao FU Jun WANG Yin-yan QI Xian-wei LIANG Guo-qing DONG Mei SHAO Zheng-bin(Department of Cardiology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Anhui Hefei 230031, China)

机构地区：[1]安徽中医药大学第一附属医院心内科,安徽合肥230031

出　　处：《安徽中医药大学学报》2017年第2期56-60,共5页Journal of Anhui University of Chinese Medicine

基　　金：安徽高校省级自然科学研究项目(kj2013z166)

摘　　要：目的从足细胞途径探讨养肝益水颗粒改善高血压肾损害的作用机制。方法将64只自发性高血压大鼠随机分4组,每组16只,即养肝益水颗粒高、低剂量治疗组,贝那普利组,模型组,并选取16只WKY大鼠作为正常组。检测实验前后各组大鼠的血清肌酐(serum creatinine,SCr)、血尿素氮(blood urea nitrogen,BUN)水平;采用RT-qPCR检测大鼠肾皮质转化生长因子(transforming growth factor beta 1,TGF-β1)、p27mRNA表达水平;Western blot检测大鼠肾皮质TGF-β1、p27蛋白表达变化。结果治疗前后各组自发性高血压大鼠SCr、BUN水平显著高于正常组(P<0.05);治疗后,养肝益水颗粒高、低剂量组,西药组大鼠SCr、BUN水平均显著降低(P<0.05),但3组之间比较,差异无统计学意义(P>0.05)。模型组肾皮质p27蛋白、p27mRNA及TGF-β1mRNA、TGF-β1蛋白表达水平均明显高于正常组(P<0.05);治疗后,养肝益水颗粒高、低剂量组相比,高剂量组肾皮质p27蛋白及p27mRNA表达水平均降低(P<0.05);肾皮质TGF-β1mRNA、TGF-β1蛋白相对表达水平显著降低(P<0.05)。结论养肝益水颗粒通过下调TGF-β1、p27的表达,调节足细胞的增殖和凋亡,维持足细胞正常形态和结构,从而达到保护肾脏的目的。domly divided into high- and low-dose Yanggan Yishui Granule groups, benazepril group, and model group, with 16 rats in each group; another 16 WKY rats were selected as normal group. The levels of serum creatinine （SCr） and blood urea nitrogen （BUN） were measured before and after the experiment, and RT-qPCR and Western blot were used to measure the mRNA and protein expression of transforming growth factor-β1 （TGF-β1） and p27 in the renal cortex in rats. Results The spontaneously hypertensive rats had significantly higher levels of SCr and BUN than the normal rats before and after treatment （P〈0.05）. After treatment, the high- and low-dose Yanggan Yishui Granule groups and the benazepril group had significant reductions in the levels of SCr and BUN （P〈0.05）, but there were no significant differences between the three groups （P〉0.05）. The model group had significantly higher mRNA and protein expression of p27 and TGF-β1 in the renal cortex than the normal group （P〈0.05）. After treatment, compared with the low-dose Yanggan Yishui Granule group, the high-dose Yanggan Yishui Granule group had significant reductions in the mRNA and protein expression of p27 and TGF-β1 （P〈0.05）. Conclusion Yanggan Yishui Granule can protect the kidney through down-regulating the expression of p27 and TGF-β1, regulating the proliferation and apoptosis of podocytes, and maintaining the normal morphology and structure of podocytes. [Key words] Yanggan Yishui Granule~ Hypertensive kidney injury; Transforming growth factor-β1 ; p27

关 键 词：养肝益水颗粒 高血压肾损害 转化生长因子β1 P27 

分 类 号：R544.1[医药卫生—心血管疾病]