基于脂质代谢网络的虎杖抗呼吸道合胞病毒肺炎代谢组学研究  

Metabonomics Study on the Effect of Polygonum Cuspidatumon Anti-respiratory Syncytial Virus(RSV) Basing on Lipid Metabolism

作　　者：谢彤[1] 杜丽娜[1] 徐建亚[1] 沈存思[1] 汪受传[1] 单进军[1] 

机构地区：[1]南京中医药大学中医儿科学研究所,江苏省儿童呼吸疾病(中医药)重点实验室,南京210023

出　　处：《世界中医药》2016年第9期1670-1673,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81303295,81373688);江苏省自然科学基金青年基金项目(编号:BK20130959);江苏省高校自然科学研究面上项目(编号:13KJB360005)

摘　　要：目的:探讨虎杖用于干预呼吸道合胞病毒(Respiratory Syncytial Virus,RSV)诱导BALB/c小鼠肺炎的脂质代谢网络的变化。方法:选取18只BALB/c小鼠,随机分为空白组、RSV肺炎组、虎杖组3组,RSV肺炎组、虎杖组滴鼻给予RSV病毒诱导产生肺炎,虎杖组灌胃给予虎杖提取物给予治疗,空白对照组灌胃给予生理盐水作为对照。实验结束,取血浆测定其血浆中的脂质代谢物质。建立基于高效液相-线性离子阱-静电场轨道杂交质谱(HPLC-LTQ-Orbitrap-MS)的脂质代谢组学技术,表征3组小鼠血浆中脂质代谢轮廓的变化,通过单因素方差分析筛选差异性化合物(P<0.0.5),并对其差异化合物进行结构确定,阐明其可能调节的脂质代谢网络。结果:多变量数据统计结果显示,3组小鼠的血浆脂质代谢轮廓区分明显,受调控的脂质分子主要为磷脂酰胆碱、三酰甘油成分和磷酸化神经酰胺。代谢通路分析结果显示,虎杖主要调节了磷脂代谢。结论:虎杖提取物通过调节磷脂代谢发挥抗RSV肺炎的作用。Objective： To observe the effect of Polygonum cuspidatum on the lipid metabolism changes of BALB / c mice caused by anti-respiratory syncytial virus. Methods： Eighteen BALB / c mice were grouped into three groups randomly,blank control group,RSV group,and Polygonum cuspidatem group. RSV group and Polygonum cuspidatem group were infected by RSV through nasal inhalation. Bland control group experienced intragastric administration of normal saline. Plasma were taken after the experiment to measure its lipid metabolism materials. And the lipids were extracted by Methyl tert-butyl ether（ MTBE） and then were introduced into HPLC-LTQ-Orbitrap for lipidomic analysis. OPLS-DA model were calculated for cluster of the three groups,and discriminated lipids were detected by One Way ANOVA（ P〈0. 05）. The structure of the discriminated lipids were determined,explaining the regulation oflipid metabolism. Results： The score plot of the OPLS-DA model provided the obvious discrimination of the three groups,suggesting the lipid metabolism regulated were phosphatidylcholine,triacylglycerol and phosphorylated ceramide. The further One Way ANOVA analysis suggested that the levels of the lyso glycerophosphocholines（ Lyso PCs） and phosphated ceramides were significantly decreased when RSV were inoculated intranasally,while the Polygonum cuspidatum can increased this changes. Conclusion： Polygonum cuspidatum potents the anti-RSV effect by regulating the lipid metabolism.

关 键 词：脂质代谢组学 虎杖 呼吸道合胞病毒肺炎 液质联用 

分 类 号：R285.5[医药卫生—中药学]