芪归银方对多重耐药铜绿假单胞菌感染大鼠血清IL-1β水平的影响  

The Impact of Qiguiyin Decoction on IL-1β in Serum of Multi-drug Resistant Pseudomonas Aeruginosa Infected Rats

作　　者：孔令博[1] 郭玉红[2] 付跃峰[1] 李艳鹏[1] 牛丽强[1] 杨雪冬[1] 姜旭[1] 刘佳莹[1] 刘清泉[2] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《世界中医药》2016年第10期1966-1969,共4页World Chinese Medicine

基　　金：北京中医药大学基本科研业务费项目(编号:2015-JYB-JSMS057);国家"十二五"科技重大专项(编号:2013ZX09102026)

摘　　要：目的:探讨芪归银方对多重耐药铜绿假单胞菌感染大鼠血清IL-1β水平的影响。方法:将SD大鼠随机分为空白组、模型组、西药对照组、芪归银方组、中西医结合组5组,运用ELISA检测采用不同干预方法的各组大鼠血清IL-1β水平,比较各组之间的差异。结果:模型组大鼠血清IL-1β水平在各时间点均较空白组明显升高,差异有统计学意义(P<0.01);西药对照组、芪归银方组、中西医结合组大鼠血清IL-1β水平在3 h、8 h、24 h较空白组明显升高,差异有统计学意义(P<0.01);西药对照组、芪归银方组、中西医结合组大鼠血清IL-1β水平在72 h、5 d较空白组无统计学意义(P>0.05);西药对照组大鼠血清IL-1β水平在72 h、5 d较模型组降低,差异有统计学意义(P<0.01);扶正透邪方组大鼠血清IL-1β水平在24 h较模型组升高,差异有统计学意义(P<0.05),在5 d较模型组降低,差异有统计学意义(P<0.01);中西医结合组大鼠血清IL-1β水平在24 h较模型组升高,在72 h、5 d较模型组降低,差异均有统计学意义(P<0.01)。结论:与抗生素相比,芪归银方能够在炎性反应早期增强促炎递质的释放,二者联用则作用更明显,有利于积极清除细菌及毒素,并随着时间的推移,使促炎递质的释放迅速下降,维持正常炎性反应水平以免太长的过激炎性反应不利于损伤的修复,充分体现了"阴平阳秘""以平为期"的中医理论。Objective： To explore the impact of qiguiyin decoction on IL-1β in serum of multi-drug resistant pseudomonas aeruginosa infection rats. Methods： SD rats were randomly divided into blank group,model group,western medicine control group,qiguiyin decoction group,and integrated Traditional Chinese Medicine and western medicine group. Level of IL-1β in rats was detected by ELISA,and then the differences between groups were compared. Results： The level of IL-1β in model group was significantly higher than that of blank group,with statistically significant difference（P 〈0. 01）. The levels of IL-1β in western medicine control group,qiguiyin decoction group,and integrated Traditional Chinese Medicine and western medicine group at 3 h、8 h、24 h were significantly higher than those of blank group,and the difference was statistically significant（P 〈0. 01）. The levels of IL-1βin western medicine control group,qiguiyin decoction group,and integrated Traditional Chinese Medicine and western medicine group at 72 h、5 d showed no statistically significant difference when comparing with those of blank group（P 〈0. 05）. The levels of IL-1β in control group at 72 h、5 d were lower than model group,the difference was statistically significant（P 〈0. 01）. The level of IL-1β in qiguiyin decoction group at 24 h was significantly higher than that of model group,and the difference was statistically significant（P 〈0. 05）. The level of IL-1β in qiguiyin decoction group at 5 d was lower than that of model group,and the difference was statistically significant（P 〈0. 01）. The level of IL-1β in integrated Traditional Chinese Medicine and western medicine group at 24 h was significantly higher than model group,and the difference was statistically significant（P 〈0. 01）. The level of IL-1β in integrated Traditional Chinese Medicine and western medicine group at 72 h、5 d were lower than model group,and the difference was statistically significant（P 〈0. 01）. Conclusion： Compared

关 键 词：扶正透邪 芪归银方 多重耐药菌感染 炎性反应因子 IL-1Β 

分 类 号：R285.5[医药卫生—中药学]