不同剂量角叉菜胶诱导血瘀证树鼩模型的研究  

Study on Tree Shrews Model of Blood Stasis Induced by Different Dose of Carrageen Glue

作　　者：陈奔[1] 黄金兰 郭茜茜[3] 冯晓桃[1] 郭尔楚 张晟瑞[3] 陈文雅[3] 何育炉 钟振国[3] 

机构地区：[1]广西中医药大学广西中医药科学实验中心,南宁530001 [2]徐州医科大学药学院,徐州221004 [3]广西中医药大学新药研究开发中心,南宁530001

出　　处：《世界中医药》2016年第11期2219-2222,共4页World Chinese Medicine

基　　金：广西科学研究与技术开发计划项目(编号:桂科攻1347003-1);广西重点实验室建设项目(编号:15-140-31;14-045-12;13-051-06);广西农作物废弃物功能成分研究协同创新中心建设(编号:CICAR2015-Z1)

摘　　要：目的:通过对不同剂量的角叉菜胶诱导建立血瘀证树鼩模型表征及血液学观察,探索由角叉菜胶诱导的血瘀证树鼩模型的最佳剂量。方法:32只树鼩随机分为对照组、低剂量组(25 mg/kg)、中剂量组(50 mg/kg)、高剂量组(75 mg/kg),8只/组,腹腔注射角叉菜胶3 d,造模24 h后肉眼结合体征采集分析系统定性定量观察表征,全自动血流变快测仪检测血液流变学指标,全自动凝血分析仪检测凝血4项。结果:造模24 h后,造模动物精神萎靡、舌底脉络增粗增长,随着剂量增加,固定性疼痛概率增多、舌质、爪部和鼻唇部皮肤颜色变深变暗。血液流变学指标中,高剂量组全血黏度、红细胞压积、红细胞聚集指数、卡松屈服应力和血浆黏度明显升高(P<0.05或P<0.01),红细胞电泳时间显著延长(P<0.01),而红细胞变形指数降低(P<0.01);中、高剂量组的APTT、PT、TT明显降低(P<0.05或P<0.01),而FIB含量增加(P<0.01)。结论:高剂量的角叉菜胶所诱导的血瘀树鼩模型成立。Objective：To explore the optimum dose in tree shrews model of blood stasis syndrome induced by carrageen glue via observation of representation and hemorheology parameters of tree shrews .Methods：A total of 32 tree shrews were randomly di-vided into 4 groups：control group, low-dose group （25 mg/kg）, middle-dose group （50 mg/kg） and high-dose group（75 mg/kg）.Eight tree shrews in each group.The drug groups were intraperitoneal injected with carrageen glue for 3 days.The represen-tation was observed by perusal and signs of collection and analysis system , hemodynamic indexes were detected by hemorrheology fast measuring instrument.The results of activated thromboplatintime （APTT）, prothrombin time（PT）, thrombin time（TT） and Fibrinogen（FIB） were measured using automated coagulation analyzer .Results：The tree shrews in drug groups were in low spir-its.Tongue vein were of enlargement of growth .The Regular probability pain increased and the colors of the tongue , claw and naso-labial area became darker with the dose increasing .Hemorheology results showed that in high-dose group, the whole blood viscosity, packed cell volume , red cell assembling index , casson yield stress and plasma viscosity increased （ P〈0.05 or P〈0.01）, erythrocyte electrophoresis time prolonged （P〈0.01）, but the RBC deformability index decreased （P〈0.01）.Com-pared to the other two groups, the APTT, PT and TT in high-dose group remarkably decreased （P〈0.05 or P〈0.01）, but FIB increased （ P〈0.01） .Conclusion：The model of blood stasis syndrome was successfully built in tree shrews with high -dose carra-geen glue .

关 键 词：角叉菜胶 血瘀证 树鼩模型 

分 类 号：R285.5[医药卫生—中药学]