自拟强心汤对心梗后心力衰竭大鼠心肌舒缩功能的实验研究  

Study on myocardial systolic and diastolic function of Qiangxin Tang on heart failure after myocardial infarction in rats

作　　者：高占义[1] 魏月娟[1] 王彦敏[2] 李二娟[1] 张姗[1] 王庆海[3] 

机构地区：[1]河北医科大学附属沧州中西医结合临床学院心内科,沧州061000 [2]河北医科大学附属沧州中西医结合临床学院超声科,沧州061000 [3]河北医科大学附属沧州中西医结合临床学院科教科,沧州061000

出　　处：《世界中医药》2016年第11期2363-2366,共4页World Chinese Medicine

基　　金：河北省中医药管理局"自拟强心汤调控CaN-NFAT信号通路干预慢性心力衰竭的临床研究"(编号:2015287)

摘　　要：目的:探讨自拟强心汤对心梗后心力衰竭大鼠心肌舒缩功能的作用机制。方法:采用结扎左冠状动脉法制备大鼠心梗后慢性心力衰竭动物模型;8周后,给予药物灌胃治疗;经治疗4周后,采用通过颈动脉插管测定大鼠血流动力学状态,通过实时荧光定量PCR(FQ-PCR)法和Western Bolt法检测心肌钙调控蛋白肌浆网钙泵(SERCA2a)、雷尼丁受体2(RYR2)mRNA水平及其蛋白表达量。结果:模型组大鼠的心功能显著下降,主要表现在血流动力学参数改变,其中左室舒张末期压(LVEDP)明显升高(P<0.01),左室收缩压(LVSP)、左室上升最大速率(+LVdp/dtmax)及左室下降最大速率(-LVdp/dtmax)绝对值均降低(P<0.05);心肌钙调控蛋白SERCA2a、RYR2 mRNA水平及其蛋白表达量均明显下降(P<0.05)。给予自拟强心汤干预后,能够有效的降低心力衰竭大鼠LVEDP水平(P<0.05),升高LVSP、+LVdp/dtmax、-LVdp/dtmax水平(P<0.01);能够升高心肌钙调控蛋白SERCA2a、RYR2 mRNA及其蛋白表达量的水平(P<0.05)。结论:自拟强心汤能够有效的改善心力衰竭大鼠的心功能,其机制可能与升高心肌钙调控蛋白SERCA2a、RYR2的蛋白表达量,改善钙循环有关。Objective：To clarify myocardial systolic and diastolic function and possible mechanism of Qiangxin Tang on the model of heart failure.Methods：Model of heart failure after myocardial infarction was prepared by left coronary artery ligation .After 8 weeks ,take a lavage treatment every day;After 4 weeks of continuous administration ,cardiac hemodynamics parameters were recor-ded and SERCA2a,RYR2 mRNA level were detected by PCR（FQ-PCR）and SERCA2a,RYR2 protein expression were detected by Western bolt.Results：compared with control group ,cardiac function of model group is lower：hemodynamic parameters LVEDP is increased obviously（P〈0.01）,LVSP,＋LVdp/dtmax and-LVdp/dtmax absolute values were all lower （P〈0.05）,and the calm-odulin SERCA2a and RYR2 mRNA and protein relative expression of model group rats was decreased （P〈0.05）.Compared with model group,Qiangxin Tang group could reduce LVEDP levels （P〈0.05）,increase LVSP,＋LVdp/dtmax and-LVdp/dtmax levels （P〈0.01 ）, and the calmodulin SERCA2a and RYR2 mRNA and protein relative expression of Qiangxin Tang were higher.Conclusion：Qiangxin Tang can effectively improves cardiac function in heart failure rats;Its mechanism is related with im-proving calmodulin SERCA2a、RYR2 and improving calcium cycle .

关 键 词：自拟强心汤 慢性心力衰竭 心肌舒缩功能 血流动力学 

分 类 号：R285.5[医药卫生—中药学]