黄芪多糖对肥胖小鼠的减肥作用与调节肠道菌群的关系研究  

Study on Anti-Obesity Effect and Modulation of Gut Microbiota by Astragalus Polysaccharides in Mice

作　　者：何旭云 贺姣姣[1] 郑宁宁[1] 王顺春[2] 李后开[1] 

机构地区：[1]上海中医药大学交叉科学研究院中医方证与系统生物学中心,上海201203 [2]上海中医药大学中药研究所,上海201203

出　　处：《世界中医药》2016年第11期2379-2384,2388,共7页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81673662);上海市科委"浦江人才"计划(编号:14PJD031);上海市教委一流学科创新项目(编号:ZYX-CXYJ-017);上海市教委"曙光学者"人才计划(2016);上海市高校特聘教授(东方学者)人才计划(2014)

摘　　要：目的:观察黄芪多糖(Astragalus Polysaccharides,APS)对于高脂饮食诱导的肥胖小鼠的减肥作用与调节肠道菌群的关系。方法:1)将50只C57bl/6J小鼠随机分为5组(n=10),分别为正常对照组(Con)、HFD(high-fat diet)组和APS低中高(在高脂饮食中添加分别添加2%,4%,或8%的APS)剂量组。利用高脂饮食连续喂养8周诱导出小鼠肥胖模型,给药组同步喂养,每周进行称重。实验周期结束后,收集各组粪便样本,利用基于细菌16S r DNA测序的元基因组学方法,分析了APS对于高脂喂养小鼠肠道菌的影响。2)将10只C57bl/6J小鼠随机分为2组(n=5),分别为HFD_R组(HFD_Receptor)和APS_R组(APS_Receptor),每天分别灌胃来自HFD组和APS低剂量组(2%APS)小鼠的新鲜粪便提取液进行肠道菌移植,前八周给予正常饮食,后四周更换为HFD。结果:APS能够明显抑制高脂喂养小鼠肥胖的形成、减轻肝脏脂肪变性、降低肝脏TG水平、改善胰岛素敏感性、显著恢复高脂喂养小鼠的肠道菌群紊乱,增加拟杆菌(Bacteroidetes)与厚壁门(Firmicutes)菌的相对丰度、降低变形菌门(Proteobacteria)细菌的相对丰度,并且,APS的减肥效应能够通过肠道菌移植转移给高脂喂养受体小鼠。结论:APS对高脂喂养小鼠具有减肥作用,且APS的减肥作用与调节肥胖小鼠的肠道菌群有关。Objective：To explore relationships between the anti-obesity effect and modulation of gut microbioata by APS in high-fat diet （HFD）-fed mice.Methods：1.A total of 50 male C57bl/6J mice were randomly divided into five groups including control group （Con）, HFD group, and three APS groups with low, middle and high dosages （2%, 4%, 8%APS mixed in HFD）.All animals were fed with corresponding diet for consecutive eight weeks during which their body weight was measured weekly .At the end of the experiment , fecal samples were collected for analysis of gut microbiota by 16S rDNA sequencing approach .2.To inves-tigate the contribution of gut microbiota to body weight reduction of APS , two groups of mice （ n=5/each group ） were transplanted with intestinal bacteria from mice that were fed with or without APS （2 % APS in HFD）.The receiver mice were designated as HFDR and APSR, respectively.The receiver mice were fed with chow diet for consecutive eight weeks first , and then switched to HFD for another four weeks .Results：APS effectively suppressed the HFD-induced obesity formation , attenuated the hepatic steatosis, decreased hepatic TG level, improved insulin sensitivity, restored the balance of gut microbiota in HFD-fed mice by in-creasing the relative abundance of Bacteroidetes and Firmicutes , and reducing the abundance of Proteobacteria bacteria .Moreover , the anti-obesity effect of APS could be transferred to mice that were transplanted with intestinal bacteria from APS -fed mice in the context of HFD , but not chow diet .Conclusion：APS could inhibit the HFD-induced obesity formation , at least partially , by modu-lating gut microbiota .

关 键 词：黄芪多糖 肥胖 肠道菌群 胰岛素敏感性 脂肪变性 16S RDNA 

分 类 号：R285.5[医药卫生—中药学]