温和灸对慢性内脏痛敏模型大鼠结肠CRFR2表达的影响  

Warming Moxibustion's Effect on Chronic Visceral Hyperalgesia and Expression of CRFR2 in Colon of Rats

作　　者：戚莉[1,2] 易韬[3] 张建斌[4] 常小荣[5] 杨玲[1] 刘慧荣[1] 丰晓溟[1] 吴焕淦[1] 

机构地区：[1]上海中医药大学上海市针灸经络研究所,上海200030 [2]上海中医药大学中医内科学E-研究院,上海201203 [3]复旦大学附属华山医院中西医结合科,上海200040 [4]南京中医药大学,南京210023 [5]湖南中医药大学,长沙410208

出　　处：《世界中医药》2016年第12期2529-2532,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81303031;81303034;81403472);国家重点基础研究发展计划("973"计划)资助项目(编号:2009CB522900);上海市针灸机制与穴位功能重点实验室资助(编号:14DZ2260500)

摘　　要：目的:观察温和灸对慢性内脏痛敏(Chronic Visceral Hyperalgesia,CVH)模型大鼠行为学及结肠组织促肾上腺皮质激素释放因子2型受体(Corticotrophin Releasing Factor Receptor 2,CRFR2)蛋白及其mRNA表达的影响。方法:采用乳鼠结直肠扩张(Colorectal Distention,CRD)刺激建立CVH大鼠模型,模型制备成功后选取双侧天枢、上巨虚穴分别进行温和灸及假灸干预,1次/d,连续7 d。全部干预结束后,以腹部撤回反射(Abdominal Withdrawl Reflex,AWR)评分作为检测大鼠内脏痛敏的行为学指标;采用免疫组织化学法、实时荧光定量PCR法观察结肠组织CRFR2蛋白及其mRNA的表达。结果:在不同强度的CRD刺激下,与正常组相比,模型大鼠AWR评分升高(P<0.01)。温和灸组干预后,大鼠AWR评分下降(P<0.01)。模型组大鼠CRFR2蛋白及其mRNA表达均高于正常组(P<0.01),温和灸干预后,CRFR2蛋白及其mRNA表达进一步升高(P<0.01)。假灸组与模型组比较,差异无统计学意义。结论:温和灸有效缓解CVH模型大鼠内脏痛敏状态,与其升高结肠组织CRFR2蛋白及其mRNA的表达有关。Objective：To observe the anti-CVH effect of warming moxibustion and its influence on the expression of CRFR2 （protein and mRNA）in the colon.Methods：CVH rat model induced by mechanical colorectal distention （CRD）and abdominal withdrawal reflex （AWR）scores were assessed by acute CRD after warming moxibustion treatment.The protein and mRNA expressions of CRFR2 in the colon were determined by immunohistochemistry and real-time PCR,respectively.Results：Warming moxibustion markedly attenuated the abdominal withdrawal reflex （AWR）scores in response to acute CRD in IBS rats;The expressions of CRFR2 （protein and mRNA）in the colon were significantly increased in the model rats than in the normal rats.Warming moxi-bustion increased the expression of CRFR2 （protein and mRNA）in the model rats.Conclusion：WM treatment may attenuate CRD-induced CVH in rats and this effect may be related to WMupregulation of colonic CRFR2.

关 键 词：温和灸 慢性内脏痛敏 结肠 促肾上腺皮质激素释放因子2型受体 大鼠 

分 类 号：R245.81[医药卫生—针灸推拿学]