茶薪菇单核与双核厚垣孢子的比较研究  

A comparative study of mononuclear chlamydospore and binuclear chlamydospore of Agrocybe chaxingu

作　　者：张筱梅[1] 朱维红[1] 张渊[1] 陈义广[1] 陈梅香[1] 张保石[1] ZHANG Xiao-mei ZHU Wei-hong ZHANG Yuan CHEN Yi-guang CHEN Mei-xiang ZHANG Bao-shi(Department of Biochemistry, Baoding University, Baoding 071000, China)

机构地区：[1]保定学院生化系,河北保定071000

出　　处：《河北农业大学学报》2017年第2期61-66,共6页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金资助项目(C2013104055)

摘　　要：以2株茶薪菇为材料,通过形态观察、杂交配对、出菇试验、耐热性比较等方法,研究了单核与双核厚垣孢子的差异,确定了其无性循环生活史。研究表明:单核孢子体积较小,呈梨形,多端生;双核孢子体积稍大,多梨形、少数葫芦状或不规则状,端生或中生;紫外辐照未发现厚垣孢子形态变化;相同培养条件下单核孢子数量较多,4~38个/视野,双核厚垣孢子耐热性较强,45℃处理8h萌发率达5.6%。Taking two samples of Agrocybe chaxingu as material, differences between mono- nuclear chlamydospore and binuclear chlamydospore are studied and the asexual life cycle is determined through phenotypic observation, which includes hybridization, germination exper- iment, and comparison of the resistance to high temperature. Results show that monokaryotic spores are comparatively small, pear-shaped, and multi-terminal; binuclear spores are com- paratively large, likely to be pear-shaped with a few of gourd-shape or irregular shape, termi- nal or mesogenous. After ultraviolet irradiation, no morphological change of chlamydospores is observed. Under the same culture conditions, the number of mononuclear chlamydospores is larger, 4-38 per field of vision. Binuclear chlamydospores are more resistant to high temper- ature, with 5.6% germination rate at 45℃ after 8 hours.

关 键 词：特征观察 耐热性 无性循环史 

分 类 号：S646[农业科学—蔬菜学]