MiR-124在脑血流灌注不足中的表达变化及其作用  

Expression and function of miR-124 in cerebral ischemia

作　　者：雷荟仔[1] 王林辉[1] 李昱[1] 

机构地区：[1]重庆医科大学基础医学院病理学教研室重庆医科大学神经科学中心重庆市神经生物学重点实验室,重庆400016

出　　处：《重庆医科大学学报》2017年第3期334-338,共5页Journal of Chongqing Medical University

摘　　要：目的:研究miR-124在脑血流灌注不足大鼠中的动态表达变化,并探讨其在脑血流灌注不足中发挥的作用。方法:采用双侧颈总动脉结扎(Permanent bilateral comman carotid artery occlusion或Two-vessel occlusion,2VO)制备大鼠脑缺血模型,用qRT-PCR的方法在不同的时间点(术后1、7、14、21、28 d)检测miR-124的表达变化。并在SH-SY5Y细胞系中采用流式细胞仪研究低氧时过表达miR-124对细胞周期的影响。结果:与对照组比较,2VO术后大鼠脑皮质血流经历了先下降再上升的动态变化,而miR-124也呈现先下降再上升的动态过程,于术后第7天达到最低(P=0.036);在低氧环境中,过表达miR-124可以将神经细胞系SH-SY5Y的阻止在G1期。结论:miR-124可能参与了大鼠脑血流灌注不足后脑缺血的发生发展,为治疗脑缺血提供了一种新的治疗策略。Objective ：To observe the change of miR-124 expression and its effect on cell cycle on cerebral ischemia in rats. Methods： The model of aging rats with cerebral hypoperfusion was successfully constructed by permanent occlusion of bilateral common carotid arteries（2VO）. The expressions of miR-124 at different time points were determined and compared by qRT- PCR. Cell cycle analysis was performed on SH-SY5Yeell line after transfection with either pre-miR-124 or scramble;cells were exposed to oxygen and glu- cose deprivation （OGD） stress at 24-48 hours,and then analyzed by fluorescence-activated cell sorting （FACS）. Results：Compared with those of control rats, the expressions of miR-124and cortical blood flowfirstlydecreased then increased, and bottomed out on the seventh dayin the 2VO rats（P=0.036）. In OGD stress ,overexpression of miR-124 in SH-SY5Y cell induced cell cycle arrested in G1 phase. Conclusion ：miR-124 is potentially involved in rat cerebral ischemia progression which may provide a novel therapeutic strategy for treatment of cerebral ischemia.

关 键 词：MIRNA 脑缺血 双侧颈总动脉结扎 

分 类 号：R651[医药卫生—外科学]