白山市鸭绿江网箱养鲤冰下爆发锦鲤疱疹病毒病的鉴定  

Identification of Koi herpesvirus disease outbreak under ice in carp cage cultured in baishan,the Yalu River

作　　者：高立容 于慧[2] 周井祥[2] Gao Lirong Yu Hui Zhou Jingxiang(Changchun city aquatic products quality and safety testing center, Changchun 130118,China College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]长春市水产品质量安全检测中心,吉林长春130033 [2]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《水产养殖》2017年第4期7-11,共5页Journal of Aquaculture

基　　金：吉林省科技发展计划疫苗研制与示范(20120228)

摘　　要：锦鲤疱疹病毒病(Kio herpesvirus disease,KHVD)是由锦鲤疱疹病毒(Kio herpesvirus,KHV)引起的鲤(Cyprinus carpio L)、锦鲤、框镜鲤的一种高传染率和致死率的疾病,发病季节多为春秋季,常发水温为18~28℃。2015年3月初,低温条件下,吉林省白山市鸭绿江某网箱养殖场鲤鱼大量死亡,其因不明。本实验采用PCR方法进行鉴定,对其编码的膜糖蛋白基因ORF25和囊膜蛋白ORF81基因进行检测,测序结果显示该病毒的目的基因片段与KHV-cj同源性达96%。综上所述,可判定该养殖场鲤爆发的疾病为锦鲤疱疹病毒病。目前该病在低温下爆发的报道还比较少,因此本实验为锦鲤疱疹病毒病爆发的温度条件提供了参考,同时也为该病的防治提出了一些措施及建议。Koi herpesvirus disease （Kio herpesvirus diseases, KHVD）is a koi herpesvirus （Kio herpesvirus, KHV） of carp （Cyprinus）, brocade carp, frame mirror carp a high infection rate and fatality rate of disease, pathogenesis season is age season more, often send water temperature for 18 to 28 ℃ In early March 2015,under the conditions of low temperature,a large amount of carp died in a cage farm in Yalu River,Baishan City, Jilin Province and the reason was unknown. In this study,the polymerase chain reaction （PCR） was used to identify the membrane glycoprotein gene ORF25 and envelope protein ORFS1 gene,the purpose of the sequencing results showed that the virus gene fragment and KHV - cj homology of 96%. In summary,it could be determine the outbreak of the farm carp disease for Koi herpes virus disease. At present,the disease has been reported at low temperature,so this experiment provides a reference for the temperature condition of Kio herpesvirus out- break, and also puts forward some measures and suggestions for the prevention and cure of the disease.

关 键 词：锦鲤疱疹病毒 网箱养殖 冰下 

分 类 号：S943[农业科学—水产养殖]