辣椒疫病拮抗菌的分离、鉴定及其生防效果  

Isolation,identification and biocontrol effect of antagonistic bacteria on Phytophthora capsici

作　　者：谈泰猛 黎继烈[1] 申爱荣[1,2] 严准[3] 张梦君[1] 祝久香 曾粮斌[3] 赵梦瑞[1] 

机构地区：[1]中南林业科技大学,长沙410004 [2]湖南省林业科学院,长沙410004 [3]中国农业科学院麻类研究所,长沙410205

出　　处：《生态学杂志》2017年第4期988-994,共7页Chinese Journal of Ecology

基　　金：中国农业科学院科技创新工程项目(CAAS-ASTIP-2015-IBFC)资助

摘　　要：从健康辣椒植株的根际土壤中分离筛选对辣椒疫病有较强抑制作用的拮抗菌,为其生防利用奠定基础。采用稀释平板涂布法和对峙培养法进行拮抗菌的分离筛选,根据菌株形态学特征、生理生化特性以及16S rDNA序列分析对其进行鉴定,通过盆栽试验检测其生防效果。结果表明:从土样中分离筛选到一株对辣椒疫病具有显著拮抗作用的细菌IBFCBF-4,IBFCBF-4对另外8种植物病原菌也有显著抑制作用;综合其形态学特征、生理生化特性以及16S rDNA序列信息,确定其为枯草芽孢杆菌(Bacillus subtilis);盆栽试验表明,IBFCBF-4对辣椒疫病防治效果为64.28%,相对于对照组,地上部分鲜重增加45.2%,根鲜重增加34.3%;IBFCBF-4对辣椒疫病防治及促生效果显著,具有良好地开发和利用价值。Bacteria were isolated and screened from the rhizosphere soil of healthy pepper to ob- tain antagonistic strains with strong inhibitory effect on Phytophthora capsici. This study aimed to provide a basis for utilization of biocontrol strains. Antagonistic strains were screened out by spread plate and dual culture method. Strains were identified by morphological, physiological and biochemical characteristics, and 16S rDNA sequences analysis. The biocontrol potential was de- termined by pot experiment. IBFCBF-4 was isolated from the soils, which showed strong inhibito- ry effect on P. capsici. And it also had strong antagonistic effect against other eight kinds of plant pathogens. This bacterium was identified as Bacillus subtilis by methods described above. Pot ex- periment showed the control efficiency was 64.28% on P. capsici, and the fresh weight of shoot and root increased by 45.2% and 34.3%, respectively, when compared with the control group. IBFCBF-4 had a strong biocontrol effect on P. capsici and a significant growth-promoting effect on pepper; it was of great development and utilization value for P. capsici control in the field.

关 键 词：辣椒疫病 枯草芽孢杆菌IBFCBF-4 筛选 鉴定 生防效果 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]